亚热带植物科学2023，Vol.52Issue(6)：511-517,7.DOI:10.3969/j.issn.1009-7791.2023.06.007
不同产地斑叶兰及近缘种植物中两种内酯苷类成分的含量测定
Determination of Lactone Glycosides in Goodyera schlechtendaliana and Its Related Species from Different Habitat
摘要
Abstract
The contents of lactone glycosides in Goodyera schlechtendaliana and its related species from different habitat were compared in this study to provide reference for the quality control of Goodyera.By HPLC-ELSD method,ChromCore AQ C18(3 μm,4.6 mm×250 mm)was used as the chromatographic column,and ultrapure water was used as the mobile phase.The flow rate was 0.5 mL·min-1,the column temperature was 30℃,and the atomization temperature of evaporative light scattering detector was 70℃.The flow rate of carrier gas was 2.5 mL·min-1,and the sample volume was 5 μL.The content of lactone glycosides in 30 batches of G.schlechtendaliana and its related species from different habitat was determined.The linear ranges of goodyeroside A and kinseoside were 62.5-1500 μg·mL-1(r=0.9995)and 62.5-1500 μg·mL-1(r=0.9996)respectively.The RSD of precision,repeatability and stability were less than 3%,and the average recovery rates were 101.41% and 101.16%respectively.The contents of goodyeroside A in 30 batches of G.schlechtendaliana and its related species were in the range of 28.31-243.07 mg·g-1,and the average content of 134.31 mg·g-1,which was quite different(RSD= 38.40%).The contents of kinseoside in 30 batches of G.schlechtendaliana and its related species were in the range of 0-184.37 mg·g-1,and the average content of 34.50 mg·g-1,and there were also great differences(RSD=141.30%).The method was simple,rapid,stable and reproducible,with good precision and accuracy,and be used for the content analysis of lactone glycosides in G.schlechtendaliana and its related species.关键词
斑叶兰/斑叶兰苷/金线莲苷/含量测定Key words
Goodyera schlechtendaliana/goodyeroside A/kinseoside/content determination分类
农业科技引用本文复制引用
敖艳霞,冯峰,陈慧玲,梁秀婷,韩玉,吴锦忠,吴岩斌,吴建国..不同产地斑叶兰及近缘种植物中两种内酯苷类成分的含量测定[J].亚热带植物科学,2023,52(6):511-517,7.基金项目
福建省卫生健康委员会中青年骨干人才培养项目(2021GGA057) （2021GGA057）
