湖北医药学院学报2024，Vol.43Issue(1)：1-6,6.DOI:10.13819/j.issn.2096-708X.2024.01.001
铁离子功能化羧甲基甲壳素基微球的制备与止血性能研究
Preparation and Hemostatic Performance of Iron-Functionalized Carboxymethyl Chitin-Based Microspheres
摘要
Abstract
Objective To explore the feasibility of using the ion-functionalized carboxymethyl chitin-based microsphere(Fe@CM-g-AA)as an absorbable hemostat.Methods The morphology of Fe@CM-g-AA microspheres were observed by op-tical microscopy and scanning electron microscopy.The CCK-8 and hemolysis assays were employed to assess the biocom-patibility of the microspheres.The in vitro enzymatic degradation experiment was carried out to evaluate the biodegradability of the microspheres.Further,the procoagulant activity of the microspheres was tested by in vitro coagulation experiments.Results Fe@CM-g-AA microspheres exhibited a spherical structure and remained stable under physiological conditions.The microspheres had low hemolysis rates(＜5%)and showed no toxicity to L929 and Lewis cells.Moreover,the micro-spheres displayed good biodegradability,with the degradation rate over 60%after 9 days.The in vitro coagulation experi-ments showed that Fe@CM-g-AA microspheres significantly reduced the clotting time from 77 s to 57 s and formed stron-ger blood clots compared with the blank control group.In addition,the microspheres shortened the activated partial throm-boplastin time(APTT)and thrombin time(TT)(P＜0.01).Conclusion Fe@CM-g-AA microspheres were prepared through electron beam irradiation and chelation.The microspheres can accelerate coagulation by promoting red blood cell ag-gregation and the cascade reaction of coagulation,demonstrating the potential value as absorbable hemostatic materials.关键词
羧甲基甲壳素/铁离子/丙烯酸/辐照接枝/止血微球Key words
Carboxymethyl chitin/Iron ion/Acrylic acid/Radiation grafting/Hemostatic microspheres引用本文复制引用
冷帆,雷赛,李留根,谢聪,韩宁,李童斐..铁离子功能化羧甲基甲壳素基微球的制备与止血性能研究[J].湖北医药学院学报,2024,43(1):1-6,6.基金项目
湖北省教育厅项目(B2022128) （B2022128）
湖北医药学院人才启动金项目(2022QDJZR006,2020QDJZR002) （2022QDJZR006,2020QDJZR002）
