湖北医药学院学报2024，Vol.43Issue(1)：13-17,5.DOI:10.13819/j.issn.2096-708X.2024.01.003
药用植物冷水花DNA条形码分析和基因组大小测定
DNA Barcoding and Genome Size Estimation of the Medicinal Plant Pilea notata
摘要
Abstract
Objective To identify the collected Pilea L.plant sample and estimate its genome size.Methods PCR amplifica-tion and sequencing were performed for the ITS2,rbcL and psbA-trnH sequences,and then sequence alignments against the Nr database were performed for species identification.With the maize Mo17 genome as the control,flow cytometry was em-ployed to estimate the genome size of this plant.Results The ITS2 sequence of collected sample showed the identity of 100%to that of Pilea notata,with an estimated genome size of 498.4 Mb.Conclusion ITS2 sequences can distinguish dif-ferent species of Pilea and flow cytometry is a convenient way to estimate plant genome size.关键词
冷水花/DNA条形码/流式细胞术/基因组大小Key words
Pilea notata/DNA barcoding/Flow cytometry/Genome size引用本文复制引用
罗湘胤,游磊,叶峥秀,张泽志,石宪铭,居婷婷,李琳,张勇洪..药用植物冷水花DNA条形码分析和基因组大小测定[J].湖北医药学院学报,2024,43(1):13-17,5.基金项目
国家自然科学基金项目(32000609) （32000609）
湖北省教育厅项目(B2021163,Q20222106) （B2021163,Q20222106）
