湖北医药学院学报2024，Vol.43Issue(1)：18-24,7.DOI:10.13819/j.issn.2096-708X.2024.01.004
蛋白磷酸酶PPM1F促进非小细胞肺癌的进展
The Protein Phosphatase PPM1F Promotes the Progression of Non-Small Cell Lung Cancer:a Review
摘要
Abstract
Objective To investigate the effects of the protein phosphatase PPM1F on the proliferation and metastasis of non-small cell lung cancer(NSCLC).Methods The vector for PPM1F knockdown was constructed,and Western blotting was employed to verify the knockdown efficiency of PPM1F.The effect of PPM1F on the function of NSCLC cells was deter-mined by the CCK-8 assay,colony formation assay,Transwell assay,invasion assay and flow cytometry.Results The plas-mid for PPM1F knockdown was successfully constructed.PPM1F knockdown inhibited the growth,proliferation,migration and invasion of NSCLC cells.When PPM1F was knocked down,the apoptosis rate of NSCLC cells increased,and the cells accumulated in the G1 phase.Conclusion PPM1F plays a role of oncogene in NSCLC.关键词
蛋白磷酸酶/PPM1F/非小细胞肺癌Key words
Protein phosphatase/PPM1F/Non-small cell lung cancer引用本文复制引用
周阳,刘家琪,柳淞,李子航,马永清,冯文筱,潘中秋,张慧美,赵红艳..蛋白磷酸酶PPM1F促进非小细胞肺癌的进展[J].湖北医药学院学报,2024,43(1):18-24,7.基金项目
湖北省科技厅基金重大项目(2022BCE012) （2022BCE012）
大学生创新创业训练项目(X202210929011) （X202210929011）
