湖北医药学院学报2024，Vol.43Issue(1)：57-60,4.DOI:10.13819/j.issn.2096-708X.2024.01.011
高效液相色谱法测定忧遁草中维生素C含量
Determination of Vitamin C in Clinacanthus Nutans Content by High Performance Liquid Chromatography
摘要
Abstract
Objective To establish a high performance liquid chromatography(HPLC)-based method for the determination of vitamin C in Clinacanthus nutans.Methods In this study,a method for the determination of vitamin C was established based on high performance liquid chromatography(HPLC),and the standard curve was established with the standard sub-stance.The linear relationship,detection limit,quantitative limit,repeatability,precision and standard addition recovery of the method were investigated.Results The established method had a good linearity within the range of 0.1 to 50.0 μg/mL,with the regression equation of y=53017x+2630.9,R2=0.999,the limit of detection of 0.06 μg/mL,the limit of quantifi-cation of 0.21 μg/mL,the relative standard deviation(RSD)of 3.80%in repeatability,the RSD of 1.49%in precision and the recovery rate of 99.50%to 102.97%.Conclusion The established method is suitable for determining the vitamin C in C.nutans,providing a reference for the development and utilization of C.nutans resources.关键词
忧遁草/HPLC/维生素C/活性成分Key words
Clinacanthus nutans/HPLC/Vitamin C/Active components引用本文复制引用
龚秋豪,杭佳瑶,覃敏,谭林玲,龙滕,屈小芹..高效液相色谱法测定忧遁草中维生素C含量[J].湖北医药学院学报,2024,43(1):57-60,4.基金项目
荆楚理工学院校级自科青年项目(QN202311) （QN202311）
大学生创新创业训练计划省级项目(S202311336043) （S202311336043）
