中国生物防治学报2024，Vol.40Issue(1)：108-116,9.DOI:10.16409/j.cnki.2095-039x.2023.01.040
绿原酸对昆虫病原线虫卡森斯氏线虫寿命及抗氧化活性的调控
Regulation of Longevity and Antioxidant Activity in Entomopathogenic Nematodes Steinernema kraussei by Chlorogenic Acid
摘要
Abstract
The effects of chlorogenic acid(CGA)on the longevity and antioxidant activity of the entomopathogenic nematodes(EPNs)Steinernema kraussei were investigated through treating S.kraussei with different concentrations of chlorogenic acid(0,50,100,500,1000 μmol/L)and then recording S.kraussei longevity,antioxidant activity,lipofuscin levels,and related genes mRNA expression levels,with the aim to explore the potential mechanisms of action of chlorogenic acid.The results showed that the maximum longevity of S.kraussei was increased by 36.36％in the treatment with100 μmol/L chlorogenic acid in comparison to the control,significantly inhibited the accumulation of lipofuscin related to aging,but increased SOD and CAT enzyme activities by 1.97 and 2.47 times,respectively.Moreover,qRT-PCR results showed that daf-16 and gst-4 genes transcription levels was increased by 1.78 and 3.51,respectively,in the nematodes fed with 100 μmol/L CGA compared with the control.However,the daf-2 gene transcription level showed a downward trend and decreased by 0.13.This study demonstrates that chlorogenic acid enhances the antioxidant activity and prolong the longevity of entomopathogenic nematodes S.kraussei by regulating the insulin/IGF-1 signaling pathway.关键词
绿原酸/昆虫病原线虫/寿命/抗氧化/胰岛素/IGF-1信号通路Key words
chlorogenic acid/entomopathogenic nematodes/lifespan/antioxidant/insulin/IGF-1 signaling pathway分类
植物保护学引用本文复制引用
陈红,李茜童,王兴铎,钱秀娟..绿原酸对昆虫病原线虫卡森斯氏线虫寿命及抗氧化活性的调控[J].中国生物防治学报,2024,40(1):108-116,9.基金项目
国家自然科学基金(31960559) （31960559）
甘肃省科技计划(21JR7RA819) （21JR7RA819）
甘肃农业大学大学生创新创业科研训练计划(202213021) （202213021）
