中国生物防治学报2024，Vol.40Issue(1)：117-125,9.DOI:10.16409/j.cnki.2095-039x.2023.02.069
恶臭假单胞菌HB3S-20在棉花中的定殖及诱导抗性研究
The Colonization and Induced Resistance of Pseudomonas putida HB3S-20 in Cotton
摘要
Abstract
Endophytes are important biocontrol resources in controlling plant diseases.The Pseudomonas putida HB3S-20 was screened as a potential biocontrol agent which had significant control effect against cotton Verticillum wilt in our laboratory.The colonization dynamics and colonization sites of strain HB3S-20 in cotton tissues were detected using rifampicin resistance labeling technique and GFP labeling technique in this study.Moreover,the activities of the plant defense-related enzymes SOD,POD and CAT of cotton after inoculation of the strain HB3S-20 were detected.The rifampicin resistance labeling experiment showed that the strain HB3S-20 mainly colonized the roots of cotton,the amount of bacteria of the strain HB3S-20 detected in cotton roots ranged from 2.2× 104 to 6.7× 104 cfu/g on the 3rd to 30th day after inoculation of the strain HB3S-20.The amount of bacteria of the strain HB3S-20 detected in cotton stems ranged from 0.04 X 104 to 2.7 X 104 cfu/g,lower than the amount of bacteria in roots,but not found in leaves.The results of GFP labeling experiment showed that the strain HB3S-20 not only attached to the surface of cotton roots but also colonized the vascular system of cotton roots.Moreover,the strain HB3S-20 could increase the activities of the plant defense-related enzymes SOD and POD in cotton,and it indicated that the strain HB3S-20 could induce systemic resistance and enhance the resistance of cotton to the pathogens.In this research,the colonization regulation and induced resistance of Pseudomonas putida HB3S-20 in cotton were studied,which provides theoretical basis for the mechanism of the strain HB3S-20 controlling cotton Verticillium wilt.关键词
恶臭假单胞菌/棉花/定殖/诱导抗性Key words
Pseudomonas putida/cotton/colonization/induced resistance分类
植物保护学引用本文复制引用
金利容,许冬,王玲,丛胜波,李文静,杨妮娜,尹海辰,黄薇,万鹏..恶臭假单胞菌HB3S-20在棉花中的定殖及诱导抗性研究[J].中国生物防治学报,2024,40(1):117-125,9.基金项目
湖北省农业科技创新中心项目(2021-620-000-001-011) （2021-620-000-001-011）
农业部华中作物有害生物综合治理重点实验室开放基金(2021ZTSJJ2) （2021ZTSJJ2）
