中国生物防治学报2024，Vol.40Issue(1)：146-156,11.DOI:10.16409/j.cnki.2095-039x.2024.02.003
生防菌ZF510的分离鉴定及其对白菜细菌性软腐病的防治效果研究
Isolation,Identification and Control Effect of Strain ZF510 on Bacterial Soft Rot of Chinese Cabbage
摘要
Abstract
Soft rot of Chinese cabbage is a serious bacterial disease caused by Pectobacterium brasiliense.Currently,,there are few effective products for the control of bacterial soft rot in vegetables.In order to screen biocontrol bacteria with good antagonistic effect against Chinese cabbage bacterial soft rot,this study isolated a strain of beneficial bacteria ZF510 from the rhizosphere soil of cabbage.Through morphological observation,physiological and biochemical characteristics,and multi-gene phylogenetic tree analysis,as well as pot experiments to verify the control effect of the antagonistic strain on Chinese cabbage bacterial soft rot.The results showed that the strain ZF510 was Pseudomonas donghuensis,which produced protease during the metabolic process,and had the ability to synthesize IAA,iron-loving and phosphorus-dissolving ability.Through the analysis of bacteriostatic spectrum,it was proved that strain ZF510 had significant antagonistic effects on seven pathogenic bacteria such as Pectobacterium brasiliensis and Xanthomonas campestris pv.campestris.The dichotomous dish method was used to determine that the volatile substances had inhibitory effects on the three pathogenic fungi of Rhizoctonia solani,Didymella bryoniae and Stemphylium solanacearum.The results of pot experiment showed that the symptoms of bacterial soft rot of Chinese cabbage inoculated with strain ZF510 were significantly reduced,and the control effect reached 44.56％.In summary,strain ZF510 has great potential for biological control and application prospects.关键词
东湖假单胞菌/白菜软腐病/巴西果胶杆菌/生物防治Key words
Pseudomonas donghuensis/cabbage soft rot/Pectobacterium brasiliense/biological control分类
农业科技引用本文复制引用
覃诗扬,陆凌晨,谢学文,石延霞,柴阿丽,王远宏,李宝聚,李磊..生防菌ZF510的分离鉴定及其对白菜细菌性软腐病的防治效果研究[J].中国生物防治学报,2024,40(1):146-156,11.基金项目
北京设施蔬菜创新团队项目(BAIC01-2023-16) （BAIC01-2023-16）
国家大宗蔬菜产业技术体系(CARS-25) （CARS-25）
中国农业科学院科技创新工程项目(CAAS-ASTIP-IVFCAAS) （CAAS-ASTIP-IVFCAAS）
农业农村部园艺作物生物学与种质创制重点实验室 （）
