中国生物防治学报2024，Vol.40Issue(1)：157-166,10.DOI:10.16409/j.cnki.2095-039x.2024.02.002
一株菜豆普通细菌性疫病拮抗细菌的分离鉴定及其生防效果
Isolation,Identification and Control Effect of Antagonistic Bacteria on Common Bacterial Blight of Common Bean
摘要
Abstract
Common bacterial blight of common bean is one of the most widespread common bean diseases in Heilongjiang Province in recent years.In severe cases,it can lead to large-scale yield reduction of common bean and affect grain quality.In order to explore the biocontrol bacteria resources of common bacterial blight of common bean,an antagonistic strain B2 with significant inhibitory ability on common bacterial blight of common bean was isolated and screened from the rhizosphere soil of healthy common bean.Based on microscopic observation,physiological and biochemical characteristics determination combined with 16S rDNA and gyrB sequence analysis,the strain B2 was identified as Bacillus velezensis.The antibacterial ring diameter of the strain B2 against Xanthomonas axonopodis pv.phaseoli(Xap)andXanthomonas fuscans subsp.fuscans(Xff)were 19.3 mm and 21.8 mm,respectively.In the pot experiment,the control efficacy of strain B2 against Xap and Xff were 55.42％and 67.77％,respectively.The determination of defense enzyme activity and malondialdehyde content in bean leaves showed that strain B2 could significantly increase the activities of SOD,POD,CAT and PPO in bean leaves and reduce the content of malondialdehyde.Its indicating that strain B2 could induce plant defense response to resist pathogens.In conclusion,strain B2 has good development and application potential in the biological control of common bacterial blight of common bean which caused by Xap and Xff..关键词
菜豆普通细菌性疫病/贝莱斯芽胞杆菌/生物防治Key words
common bacterial blight of common bean/Bacillus velezensis/control efficacy分类
农业科技引用本文复制引用
刘明,王昕,高涵,郭永霞..一株菜豆普通细菌性疫病拮抗细菌的分离鉴定及其生防效果[J].中国生物防治学报,2024,40(1):157-166,10.基金项目
黑龙江省"双一流"新一轮建设学科协同创新成果建设项目(LJGXCG2022-107) （LJGXCG2022-107）
国家重点研发计划(2020YFD1001402) （2020YFD1001402）
