中国临床药理学与治疗学2024，Vol.29Issue(3)：241-251,11.DOI:10.12092/j.issn.1009-2501.2024.03.001
UPLC-QE-MS联合网络药理学分析补中益气丸治疗溃疡性结肠炎的机制及实验验证
UPLC-QE-MS combined network pharmacological analysis of the mechanism of ulcerative colitis
摘要
Abstract
AIM:To explore the anti-ulcerative colitis mechanism of Buzhongyiqi pills based on the network pharmacology and experimental verifica-tion.METHODS:UPLC-QE-MS was used for qualita-tive analysis of Buzhongyiqi pills.Targets of the chemistry constituents and the disease were re-trieved from GeneCards.Then the protein-protein interaction(PPI)network was constructed and core targets were screened for GO term enrichment and KEGG pathway enrichment.Ulcerative colitis mouse model was established to verify the key tar-gets.RESULTS:A total of 41 constituents migrating of Buzhongyiqi pills were identified.A total of 123 common targets of the constituents and the dis-ease and 24 core targets were screened out.KEGG enrichment and PPI network analysis showed that Buzhongyiqi pills may play a role in the treat-ment of ulcerative colitis through Akt,PI3K and oth-er pathways.Furthermore,the results of animal ex-periments showed that Buzhongyiqi pills could sig-nificantly improve the depression behaviors of ul-cerative colitis,reduce the levels of IL-6 and TNF-αin serum,inhibition Akt/PI3K signaling,and reduce the protein expression of PI3K.CONCLUSION:Bu-zhongyiqi pills may play a role in the treatment of ulcerative colitis by inhibition Akt/PI3K signaling pathway,and inhibiting PI3K and reduce the levels of IL-6 and TNF-α in the mice.关键词
补中益气丸/网络药理学/UPLC-QE-MS/溃疡性结肠炎/KEGGKey words
Buzhongyiqi pills/network pharma-cology/UPLC-QE-MS/ulcerative colitis/KEGG分类
医药卫生引用本文复制引用
苏欣,罗琳..UPLC-QE-MS联合网络药理学分析补中益气丸治疗溃疡性结肠炎的机制及实验验证[J].中国临床药理学与治疗学,2024,29(3):241-251,11.基金项目
国家自然科学基金面上项目(82170544) （82170544）
南通市基础科学研究计划项目(JC2020039) （JC2020039）
