UPLC-QE-MS联合网络药理学分析补中益气丸治疗溃疡性结肠炎的机制及实验验证OA北大核心CSTPCD

AIM:To explore the anti-ulcerative colitis mechanism of Buzhongyiqi pills based on the network pharmacology and experimental verifica-tion.METHODS:UPLC-QE-MS was used for qualita-tive analysis of Buzhongyiqi pills.Targets of the chemistry constituents and the disease were re-trieved from GeneCards.Then the protein-protein interaction(PPI)network was constructed and core targets were screened for GO term enrichment and KEGG pathway enrichment.Ulcerative colitis mouse model was established to verify the key tar-gets.RESULTS:A total of 41 constituents migrating of Buzhongyiqi pills were identified.A total of 123 common targets of the constituents and the dis-ease and 24 core targets were screened out.KEGG enrichment and PPI network analysis showed that Buzhongyiqi pills may play a role in the treat-ment of ulcerative colitis through Akt,PI3K and oth-er pathways.Furthermore,the results of animal ex-periments showed that Buzhongyiqi pills could sig-nificantly improve the depression behaviors of ul-cerative colitis,reduce the levels of IL-6 and TNF-αin serum,inhibition Akt/PI3K signaling,and reduce the protein expression of PI3K.CONCLUSION:Bu-zhongyiqi pills may play a role in the treatment of ulcerative colitis by inhibition Akt/PI3K signaling pathway,and inhibiting PI3K and reduce the levels of IL-6 and TNF-α in the mice.