中国生物防治学报2024，Vol.40Issue(1)：206-218,13.DOI:10.16409/j.cnki.2095-039x.2023.02.072
禾谷镰刀菌Fgβ2 S138A对多菌灵和噻菌灵敏感性的影响
Fgβ2 S138 A Affects the Sensitivity of Fusarium graminearum to Carbendazim and Thiabendazole
摘要
Abstract
Fusarium head blight(FHB)caused by Fusarium graminearum is a devastating disease of wheat,barley,oats,rye,and other cereal crops.Currently,the control of FHB in production mainly depends on chemical agents,among which carbendazim and other benzimidazole fungicides are widely used.The target of these fungicides is β-tubulin.There are two β tubulins in F.graminearum.A previous study showed that the 138th amino acid of β2-tubulin might be a carbendazim binding site.In this study,a Fgβ2 Ser138 mutation was constructed to clarify its biological function.The results showed that the sensitivity of Fgβ2 S138A mutation to carbendazim was significantly increased,and the EC50 value decreased from 0.617 mg/L to about 0.290 mg/L,however,the mutations did not affect the sensitivity to thiabendazole,and the EC50 value was about 0.950 mg/L,and all of them did not affect the vegetative growth,asexual reproduction,sexual reproduction and pathogenicity of F.graminearum.These results provide a theoretical basis for the chemical control of carbendazim against FHB and have a particular guiding significance in production.关键词
禾谷镰刀菌/小麦赤霉病/药剂敏感性/定点突变Key words
Fusarium graminearum/Fusarium head blight/fungicide sensitivity/site-directed mutagenesis分类
农业科技引用本文复制引用
蒋凡,吕俊博,赵彦翔,张迎新,黄金光..禾谷镰刀菌Fgβ2 S138A对多菌灵和噻菌灵敏感性的影响[J].中国生物防治学报,2024,40(1):206-218,13.基金项目
国家自然科学基金(31871916) （31871916）
国家重点研发项目(2022YFD1400104) （2022YFD1400104）
