中国肺癌杂志2024，Vol.27Issue(2)：109-117,9.DOI:10.3779/j.issn.1009-3419.2024.102.01
肺腺癌中FAT1的表达及其与免疫细胞浸润关系的研究
Expression of FAT1 in Lung Adenocarcinoma and Its Relationship with Immune Cell Infiltration
摘要
Abstract
Background and objective Lung cancer is a leading cause of cancer-related deaths.Non-small cell lung cancer(NSCLC)is the most common pathological subtype,with adenocarcinoma being the predominant type.FAT atypical cadherin 1(FAT1)is a receptor-like protein with a high frequency of mutations in lung adenocarcinoma.The protein encoded by FAT1 plays a crucial role in processes such as cell adhesion,proliferation,and differentiation.This study aims to investigate the expression of FAT1 in lung adenocarcinoma and its relationship with immune infiltration.Methods Gene expression levels and relevant clinical information of 513 lung adenocarcinoma samples and 397 adjacent lung samples were obtained through The Cancer Genome Atlas(TCGA)and Genotype-Tissue Expression(GTEx)data.The mRNA expression levels of the FAT1 gene in lung adenocarcinoma tissues were analyzed,along with its association with the prognosis of lung adenocarcinoma patients.Pathway enrichment analysis was conducted to explore the signaling pathways regulated by the FAT1 gene.Immu-noblotting was used to detect the differential expression of FAT1 in lung epithelial cells and various lung cancer cell lines,while immunohistochemistry was employed to assess FAT1 expression in lung cancer and adjacent tissues.Results FAT1 gene muta-tions were identified in 14%of lung adenocarcinoma patients.TCGA database data revealed significantly higher FAT1 mRNA expression in lung adenocarcinoma tissues compared to adjacent lung tissues.Kaplan-Meier analysis indicated lower survival rates in lung adenocarcinoma patients with higher FAT gene expression.Pathway enrichment analysis suggested the involve-ment of FAT1 in tumor development pathways,and its expression was closely associated with immune cell infiltration.Immu-nohistochemical validation demonstrated significantly higher expression of FAT1 in cancer tissues compared to adjacent lung tissues.Conclusion FAT1 mRNA is highly expressed in lung adenocarcinoma tissues,and elevated FAT1 mRNA expression is associated with poor prognosis in lung adenocarcinoma patients.FAT1 may serve as a potential biomarker for lung cancer.关键词
肺肿瘤/FAT1/预后/增殖/免疫Key words
Lung neoplasms/FAT1/Prognosis/Proliferation/Immune引用本文复制引用
丁晨,刘红雨,陈军,赵文浩,黄华,李永文,张展瑞,张芮豪,王亚楠,吴迪,陈琛..肺腺癌中FAT1的表达及其与免疫细胞浸润关系的研究[J].中国肺癌杂志,2024,27(2):109-117,9.基金项目
本研究受国家自然科学基金项目(No.82172569、No.82072595和No.61973232)、天津市医学重点学科(特色)建设项目(No.TJYXZDXK-061B)、天津市卫生科技项目(No.TJWJ2022XK005、No.5ZC20179)和北京市科技创新医学发展基金项目(No.KC2021-JX-0186-57)资助 This study was supported by the grants from National Natural Science Foundation of China(No.82172569,to Yon-gwen LI （No.82172569、No.82072595和No.61973232）
No.82072595,No.61973232,both to Jun CHEN),Tianjin Key Medical Discipline(Specialty)Construction Project(No.TJYXZDXK-061B)(to Jun CHEN),Tianjin Health Science and Technology Project(No.TJWJ2022XK005,to Jun CHEN （Specialty）
No.5ZC20179,to Hongyu LIU)and Beijing Science and Technology Innovation Medical Development Fund(No.KC2021-JX-0186-57)(to Jun CHEN). （No.KC2021-JX-0186-57）
