皖南蝮蛇毒抑瘤组分-Ⅰ对胃癌MKN-28细胞增殖、迁移及凋亡的影响OA北大核心CSTPCD

AIM:To investigate the effects of ag-kis-trodon halys venom anti-tumor component Ⅰ(AHVAC-Ⅰ)on the biological behavior of gastric can-cer MKN-28 cells.METHODS:Gastric cancer MKN-28 cells were treated with the experimental con-centrations(5,10,15 μg/mL)of AHAVC-Ⅰ for 24 h.Cell proliferation and toxicity assay(cell counting kit-8,CCK-8)was used to detect the inhibition rates of the cells in different concentrations of AHVAC-Ⅰ.The migration ability of the cells was evaluated by wound-healing and Transwell assay.The apoptosis were observed by laser confocal microscopy with annexin V-mCherry/DAPI double staining,and the apoptosis rates were analyzed by flow cytometry with annexin V-FITC/PI double fluorescence stain-ing.The protein level of Caspease-3 was deter-mined by Western blot.RESULTS:Compared with normal control group,the results of AHVAC-Ⅰ con-centration groups showed that with the increase of AHVAC-Ⅰ concentration,the proliferative activity of MN-28 cells decreased gradually(P＜0.01),the cell migration ability decreased gradually(P＜0.01),and the cell apoptosis rate increased(P＜0.05).The ex-pression of apoptosis-related protein Caspease-3 was up-regulated(P＜0.01).CONCLUSION:AHVAC-Ⅰinhibits proliferation and migration of gastric can-cer MSN-28 cells and induces apoptosis.