中国临床药理学与治疗学2024，Vol.29Issue(3)：270-276,7.DOI:10.12092/j.issn.1009-2501.2024.03.004
皖南蝮蛇毒抑瘤组分-Ⅰ对胃癌MKN-28细胞增殖、迁移及凋亡的影响
Effects of the proliferation,migration and apoptosis of AHVAC-Ⅰ on gastric cancer MKN-28 cells
摘要
Abstract
AIM:To investigate the effects of ag-kis-trodon halys venom anti-tumor component Ⅰ(AHVAC-Ⅰ)on the biological behavior of gastric can-cer MKN-28 cells.METHODS:Gastric cancer MKN-28 cells were treated with the experimental con-centrations(5,10,15 μg/mL)of AHAVC-Ⅰ for 24 h.Cell proliferation and toxicity assay(cell counting kit-8,CCK-8)was used to detect the inhibition rates of the cells in different concentrations of AHVAC-Ⅰ.The migration ability of the cells was evaluated by wound-healing and Transwell assay.The apoptosis were observed by laser confocal microscopy with annexin V-mCherry/DAPI double staining,and the apoptosis rates were analyzed by flow cytometry with annexin V-FITC/PI double fluorescence stain-ing.The protein level of Caspease-3 was deter-mined by Western blot.RESULTS:Compared with normal control group,the results of AHVAC-Ⅰ con-centration groups showed that with the increase of AHVAC-Ⅰ concentration,the proliferative activity of MN-28 cells decreased gradually(P＜0.01),the cell migration ability decreased gradually(P＜0.01),and the cell apoptosis rate increased(P＜0.05).The ex-pression of apoptosis-related protein Caspease-3 was up-regulated(P＜0.01).CONCLUSION:AHVAC-Ⅰinhibits proliferation and migration of gastric can-cer MSN-28 cells and induces apoptosis.关键词
AHVAC-Ⅰ/胃癌/细胞增殖/细胞迁移/细胞凋亡Key words
agkis-trodon halys venom antitumor component-Ⅰ(AHVAC-Ⅰ)/gastric cancer/cell prolifer-ation/cell migration/cell apoptosis分类
医药卫生引用本文复制引用
黄小梅,支慧,陈浩,卢林明,朱晓群,王丽珍,周珏,庞金金,许金亮..皖南蝮蛇毒抑瘤组分-Ⅰ对胃癌MKN-28细胞增殖、迁移及凋亡的影响[J].中国临床药理学与治疗学,2024,29(3):270-276,7.基金项目
安徽省教育厅自然科学研究重点项目(KJ2020A0611) （KJ2020A0611）
皖南医学院大学生科研资助金项目(WK2020S01) （WK2020S01）
活性生物大分子研究安徽省重点实验室自主研究课题(LAB202004) （LAB202004）
安徽省高等学校质量工程项目(S202010368107) （S202010368107）
