中国兽医科学2024，Vol.54Issue(2)：151-156,6.DOI:10.16656/j.issn.1673-4696.2024.0037
牛传染性鼻气管炎病毒微滴数字PCR检测方法的建立与初步应用
Establishment and preliminary application of droplet digital PCR detection method for bovine infectious rhinotracheitis virus
摘要
Abstract
To establish a droplet digital PCR(ddPCR)method for the detection of infectious bovine rhinotracheitis virus(IBRV),this study designed specific primers and probes for IBRV based on the re-al-time fluorescent quantitative PCR(qPCR)detection method.The specificity,sensitivity,and repeata-bility of the method were evaluated,followed by testing clinical samples.The results showed that the method could detect all IBRV strains and the results of other bovine disease pathogens were negative in the specificity test.The minimum detection limit was 4 copies/μL,with good repeatability and a coef-ficient of variation of 0.70％.Using the ddPCR method,37 samples of bovine serum,liver,lung,spleen,intestines,trachea,and fecal swabs collected and stored in our institution were tested.The detection results were consistent with clinical test results.Therefore,the droplet digital PCR method estab-lished in this study provides technical support for early detection of infectious bovine rhinotra-cheitis and qualitative and quantitative detection of bovine serum and beef food safety.关键词
牛传染性鼻气管炎病毒/微滴式数字PCR/定性定量检测Key words
bovine infectious rhinotracheitis virus/droplet digital PCR/qualitative and quanti-tative detection分类
农业科技引用本文复制引用
吴顺,程悦宁,罗国良,商金源,闫曼平,王春霞,冯二凯,易立,王振军,赵艳,郭利..牛传染性鼻气管炎病毒微滴数字PCR检测方法的建立与初步应用[J].中国兽医科学,2024,54(2):151-156,6.基金项目
中国农业科学院科技创新工程项目(CAAS-ASTIP-2021-ISAPS) （CAAS-ASTIP-2021-ISAPS）
吉林省科技发展计划项目(20210202101NC) （20210202101NC）
2023年省预算内基本建设资金项目(2023C029-6) （2023C029-6）
