N1亚型猪流感病毒荧光定量PCR检测方法的建立及初步应用OA北大核心CSTPCD
Establishment and preliminary application of a real-time PCR method for detecting N1 subtype swine influenza virus
为建立一种灵敏准确检测N1亚型猪流感病毒(SIV)的SYBR Green实时荧光定量PCR方法,本研究靶向N1亚型SIV NA基因的保守区设计特异性引物并构建质粒标准品,通过优化反应条件建立real-time PCR检测方法,对其特异性、敏感性、重复性进行评估,与常规PCR方法进行对比并应用于临床检测.结果显示,优化后所建方法的标准曲线为y=-3.329 7x+36.881,R2=0.999 5,E=99.6％,质粒标准品的拷贝数与Ct值具有良好…查看全部>>
In order to establish a sensitive and accurate SYBR Green real-time PCR method for the detection of N1 subtype SIV,specific primers were designed with the conserved region of the N1 subtype SIV NA gene as target gene,and plasmid standards were constructed.A real-time PCR assay was developed by optimizing the reaction conditions and evaluating its specificity,sensitivity and reproducibility.This method was compared with conventional PCR method and applied to …查看全部>>
张傲;王佳慧;王文佳;谭斌;张淑琴
中国农业科学院特产研究所,吉林长春 130112中国农业科学院特产研究所,吉林长春 130112中国农业科学院特产研究所,吉林长春 130112中国农业科学院特产研究所,吉林长春 130112中国农业科学院特产研究所,吉林长春 130112
畜牧业
猪流感病毒N1亚型实时荧光定量PCR鉴别诊断
swine influenza virusN1 subtypereal-time PCRdifferential diagnosis
《中国兽医科学》 2024 (2)
157-161,5
吉林省重点研发项目(20220202058NC)吉林省创新创业人才项目(2021Y034)
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