鸽圆环病毒TaqMan荧光定量PCR检测方法的建立及初步应用OA北大核心CSTPCD
Establishment and application of PCR assay for detect of pigeon circovirus
为建立快速灵敏检测鸽圆环病毒(PiCV)的方法,参考GenBank上给出的中国流行PiCV毒株序列(登录号:OL901205.1),合成了 PiCV的全基因组重组质粒,并依据PiCV的Rep基因保守序列设计多组引物、探针,从中优选出1组扩增效果最佳的引物、探针,建立TaqMan荧光定量PCR检测方法.结果显示,该方法具有良好的灵敏性,使用合成的标准重组质粒进行试验,最低检测限为21 copies/μL,且在标准质粒浓度2.1 × 108～2.1 …查看全部>>
To establish a method for the rapid and sensitive detection of pigeon circovirus(PiCV),a genome-wide recombinant plasmid of PiCV had been constructed according to a PiCV popular strain genome sequence(GenBank:OL901205.1).Subsequently,multiple sets of primers and probes were designed based on the conserved sequence of PiCV Rep gene.After screening,the optimized primers and probes com-bination which presented the best amplification efficiency was selected.And …查看全部>>
郭学波;赵承锋;吴瑶;吴琼;胡学文
汉唐和元(武汉)科技有限公司,湖北武汉 430070安陆市动物疫病预防控制中心,湖北孝感 432600汉唐和元(武汉)科技有限公司,湖北武汉 430070汉唐和元(武汉)科技有限公司,湖北武汉 430070汉唐和元(武汉)科技有限公司,湖北武汉 430070
畜牧业
鸽圆环病毒TaqMan荧光定量PCR检测应用
pigeon circovirusTaqMan fluorescence PCRdetectionapplication
《中国兽医科学》 2024 (2)
169-173,5
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