中国兽医科学2024，Vol.54Issue(2)：185-192,8.DOI:10.16656/j.issn.1673-4696.2024.0003
四株水貂肠炎病毒的分离鉴定及遗传进化分析
Isolation,identification and phylogenetic analysis of four strains of mink enteritis viruses
摘要
Abstract
In order to investigate the prevalence of mink enteritis virus(MEV)in China,clinical samples of mink suspected of mink viral enteritis from Shandong Province were subject to testing for MEV detection and isolation.The intestinal contents of mink that were positive for MEV by PCR were isolated for MEV using F81 cells.Four MEV isolates,MEV SD-1,MEV SD-4,MEV SD-5,and MEV SD-6,were identified through electron microscopy morphology observation,serology,molecular biology,and sequencing.The genomes of the four viruses were sequenced and analyzed,and the homology analysis of the main protein amino acid sequences between the isolates and the reference strains was carried out.The results showed that the isolates share 99.5％-99.7％and 99％-99.9％amino acid sequence identities with each other in VP2 and NS1 proteins,respectively,and that was 96.5％-99.7％and 98.4％-99.6％with the 32 reference strains deposited in GenBank.This study laid a foundation for the epidemiology,development of vaccines,and establishment of diagnostic methods for MEV.关键词
水貂肠炎病毒/分离/鉴定/遗传进化分析Key words
mink enteritis virus/isolation/identification/phylogenetic analysis分类
农业科技引用本文复制引用
孟祥书,程悦宁,王建科,陈汉,郭杰,姚梦凡,和彦良,陈超阳,孙见,李丽敏,刘聚祥..四株水貂肠炎病毒的分离鉴定及遗传进化分析[J].中国兽医科学,2024,54(2):185-192,8.基金项目
国家自然科学基金项目(32373001) （32373001）
河北农业大学引进人才科研专项资助项目(YJ2021019) （YJ2021019）
河北省省属高等学校基本科研业务费资助项目(KY2021010) （KY2021010）
