中国兽医科学2024，Vol.54Issue(2)：193-199,7.DOI:10.16656/j.issn.1673-4696.2024.0026
云南库蠓源蓝舌病毒血清5型的分离与鉴定
Isolation and identification of bluetongue virus serotype 5 from Culiocides in Yunnan of China
摘要
Abstract
To investigate the prevalence of bluetongue virus(BTV)in Culicoides from Xishuangbanna,Yun-nan province,Culicoides were collected from Menghai and Jinghong in Xishuangbanna from July to August 2022,and bluetongue virus was isolated and identified.Bluetongue virus in Culicoides was detected by qRT-PCR.Among 127 test samples,3 were BTV positive,resulting in a positive rate of 2.36％.These posi-tive samples were inoculated into C6/36 cells for continuous passage,and the third-generation isolat-ed cultures exhibited a significant cytopathic effect(CPE).The isolated cultures were confirmed to con-tain BTV by qRT-PCR,and the isolates were all BTV-5 based on the sequence analysis of L2 gene.These L2 gene sequences of the 3 isolates shared more than 93％similarity with the reference BTV-5 strain(AJ585126.1)and over 98％similarity with the BTV-5 strain(KT945045.1)isolated from Shizong,Yunnan province.The simi-larity of the L3 gene sequence from the 3 isolates with BTV-4(JX560415.1)isolated from Mangshi of Yunnan province was more than 96.8％.These results underscore the prevalence of BTV-5 in Culicoides in Xishuangbanna,Yunnan province.This study provided data for the study of bluetongue virus vector and the prevention and control of bluetongue disease.关键词
库蠓/蓝舌病毒/血清5型/分离/鉴定Key words
Culicoides/bluetongue virus/serotype 5/isolation/identification分类
农业科技引用本文复制引用
李苏胜,杨清荣,何于雯,杨振兴,孟锦昕,王静林,高洪..云南库蠓源蓝舌病毒血清5型的分离与鉴定[J].中国兽医科学,2024,54(2):193-199,7.基金项目
国家自然科学基金项目(32260896) （32260896）
中央引导地方科技发展资金项目(202207AB110006) （202207AB110006）
国家重点研发计划项目(2022YFC2601603) （2022YFC2601603）
云南省外国人才引进专项-重点外国专家项目(202305A0350020) （202305A0350020）
云南省热带亚热带动物病毒病重点实验室自主研究项目(2022RW007) （2022RW007）
