中国兽医科学2024，Vol.54Issue(2)：200-207,8.DOI:10.16656/j.issn.1673-4696.2024.0012
PRRSV BZ4-19株的分离鉴定及其全基因序列分析
Isolation,identification and complete gene sequence analysis of PRRSV BZ4-19 strain
摘要
Abstract
To understand the prevalence and variation of PRRSV in a pig farm in Binzhou,Shandong Province,China,suspected PRRSV-positive material collected from this farm was subjected to RT-PCR.The detected positive samples were isolated using Marc-145 cells,then nine pairs of amplification primers were designed for whole gene amplification of the isolate(named BZ4-19),and the amplified whole gene sequence was analyzed for nucleotides and their deduced amino acid sequences.The results showed that a RespPRRS MLV-like strain was successfully isolated from Marc-145 cells,and the whole gene am-plification showed that its full length was 15 412 bp(without polyA).Nucleotide homology analysis showed that it had the highest homology with the American classical strain VR2332 and RespPRRS MLV(vaccine strain),with 99.4％and 99.8％.Amino acid sequences of BZ4-19 isolates were analyzed in comparison with those of VR2332,RespPRRS MLV,the results showed that VR2332 and RespPRRS MLV had a total of 30 amino acid differences.Of these 30 differences,the isolate was consistent with VR2332 in 7 instances and with RespPRRS MLV in 23 instances.In the identification of nine amino acid sites that may be related to virulence between VR2332 and RespPRRS MLV,seven of the isolates were identical to VR2332.It is hy-pothesized that the strain isolated in this study belongs to the vaccine strain RespPRRS MLV returned strong strain,and the prevalence of PRRSV with the above molecular changes in China should be con-cerned.关键词
猪繁殖与呼吸障碍综合征病毒(PRRSV)/经典美洲株/RespPRRS MLV株/病毒分离鉴定/流行病学Key words
porcine reproductive and respiratory syndrome virus(PRRSV)/classical American strain/RespPRRS MLV strain/virus isolation and characterization/epidemiology分类
农业科技引用本文复制引用
李思琪,刘莹,刘濮毓,任晓祥,李岩岩,王文钊,左玉柱,范京惠..PRRSV BZ4-19株的分离鉴定及其全基因序列分析[J].中国兽医科学,2024,54(2):200-207,8.基金项目
河北省农业产业技术体系生猪创新团队项目(HBCT2024220201,HBCT2024220401) （HBCT2024220201,HBCT2024220401）
石家庄市科技计划项目(229500232N) （229500232N）
