中国兽医科学2024，Vol.54Issue(2)：248-254,7.DOI:10.16656/j.issn.1673-4696.2024.0021
牛呼吸道合胞体病毒检测方法的研究进展
Research progress in detection methods for bovine respiratory syncytial virus
摘要
Abstract
Bovine respiratory syncytiosis is a respiratory disease caused by bovine respiratory syncytial virus(BRSV),which is an acute,febrile and highly infectious disease;BRSV has high infection rate,low mortali-ty,and often secondary bacterial infection.The seropositive rate of BRSV in China is 41.2％-94.4％.In view of the lack of effective vaccines and drugs for BRSV in China,in order to control the transmission and efficient diag-nosis of BRSV,and to facilitate various veterinary institutions to select appropriate detection methods,in this paper the isolation and identification of BRSV,RT-PCR,fluorescent quantitative RT-PCR,nano RT-PCR,re-verse transcription isothermal isolation RT-PCR(iiRT-PCR),nested PCR,loop mediated isothermal amplifiica-tion(LAMP),genome sequencing technology,immunohistochemistry(IHC),direct immunofluorescence(D-FAT),virus neutralization test(VNT),indirect ELISA,double antibody sandwich ELISA(DAS-ELISA)were reviewed and compared.关键词
牛呼吸道合胞体病毒/分离鉴定/检测方法Key words
bovine respiratory syncytium virus/isolation and identification/test method分类
农业科技引用本文复制引用
白和平,史秋梅,王亚男,王晓丹..牛呼吸道合胞体病毒检测方法的研究进展[J].中国兽医科学,2024,54(2):248-254,7.基金项目
中央引导地方科技发展资金项目(河北省奶肉牛呼吸道疾病综合征诊断与防控技术研究,236Z6604G) （河北省奶肉牛呼吸道疾病综合征诊断与防控技术研究,236Z6604G）
河北省现代农业产业技术体系肉牛产业创新团队疫病防控与减抗岗位项目(HBCT2023190201) （HBCT2023190201）
河北省重点研发计划乡村振兴技术创新专项(犊牛绿色高效养殖与环境调控技术研究与示范,22326612D) （犊牛绿色高效养殖与环境调控技术研究与示范,22326612D）
