中国生物防治学报2023，Vol.39Issue(6)：1434-1445,12.DOI:10.16409/j.cnki.2095-039x.2023.02.068
异辛醇对灰葡萄孢的抑菌作用及转录组分析
Inhibition Effect and Transcriptome Analysis of Isooctyl alcohol on Botrytis cinerea
摘要
Abstract
To reveal the inhibitory mechanism of isooctyl alcohol to Botrytis cinerea,the inhibition effect of isooctyl alcohol on B.cinerea was determined using two-sealed-base-plate method.Mycelial morphology treated with isooctyl alcohol was observed by scanning electron microscopy.The influence of isooctyl alcohol on the extracellular conductivity and the ergosterol synthesis were measured.Response of B.cinerea to isooctyl alcohol was analyzed by RNA sequencing(RNA-Seq).The results showed that the inhibition rate of isooctyl alcohol to mycelia was 67.92％.After treatment with isooctyl alcohol,lots of mycelia were deformed and distorted with the increasing of membrane permeability.At 4,5 and 6 days after isooctyl alcohol treatment,the ergosterol contents in cell membrane decreased to 44.35％,38.82％and 42.43％,and the protein concentration decreased to 65.70％,77.93％and 58.57％,respectively.A total of 577 differentially expressed genes(DEGs),including 312 up-regulated genes and 265 down-regulated genes,were obtained by RNA-Seq.Based on functional analysis of clusters of orthologous groups(COG),gene ontology(GO)and kyoto encyclopedia of genes and genomes(KEGG),DEGs involved in membrane integral components,ergosterol biosynthesis pathway,amino acid metabolism and ribosome biogenesis pathway were significantly down-regulated,indicating that isooctyl alcohol might affect ergosterol content,energy production and ribosome structure,and further play inhibit role in the growth of B.cinerea.关键词
异辛醇/灰葡萄孢/抑菌作用/转录组Key words
isooctyl alcohol/Botrytis cinerea/inhibition effect/RNA-Seq分类
农业科技引用本文复制引用
段天坤,王燕,袁佳琪,苏雅馨,史璐欣,王琳,苏健,王美琴,王春伟..异辛醇对灰葡萄孢的抑菌作用及转录组分析[J].中国生物防治学报,2023,39(6):1434-1445,12.基金项目
山西农业大学植物保护学院科研培育创新项目(ZBXY23B-1,ZBXY23B-3) （ZBXY23B-1,ZBXY23B-3）
山西省基础研究计划(自由探索类)面上项目(20210302123404,20210302123405) （自由探索类）
山西省研究生教育创新项目(2022Y346) （2022Y346）
山西省现代农业产业技术体系建设专项资金(2021-11) （2021-11）
山西农业大学"特""优"农业高质量发展科技支撑工程(TYGC-09) （TYGC-09）
山西农业大学科技创新基金项目(2016YJ11,2016YJ12) （2016YJ11,2016YJ12）
