中国兽医科学2024，Vol.54Issue(2)：267-275,9.DOI:10.16656/j.issn.1673-4696.2024.0025
MEK1/2-ERK1/2通路参与硒在高皮质醇水平下对山羊子宫内膜炎症反应的抑制作用
MEK1/2-ERK1/2 pathway participates in the inhibition of inflammation of goat endometrium with high cortisol level
摘要
Abstract
To investigate the effect of selenium on the MEK1/2-ERK1/2 pathway in goat endometrium under a high cortisol level,the uterine infection model of dairy goats was established by intrauterine infusion of Escherichia coli.Additionally,the animal received intramuscular injection of cortisol and dietary supplementation with yeast selenium.The changes in the physiological indicators,the endometrial histology,the uterine secretion,and the MEK1/2-ERK1/2 pathway were detected.As a result,the administration of cortisol for 7 consecutive days maintained a high serum cortisol level.The supplementation of selenium for 21 days stably elevated the serum selenium level.Goats received E.coli treatment presented increased respiratory rate,heart rate,body temperature,peripheral white blood cell counts,and polymorphonuclear neutrophil(PMN)amount in uterine secretions,the endometrial damage,and the activation of MEK1/2-ERK1/2 pathway.Cortisol treatment ameliorated the changes in the physiological indicators,inflammation markers,and tissue injury,and reduced the gene and protein expressions related to MEK1/2-ERK1/2 pathway(P＜0.05).In goats received dietary supplementation with yeast selenium,there was a further inhibition in the physiological indicators,the PMN amount in uterine secret ions,the uterine tissue damage,and the MEK1/2-ERK1/2 activation(P＜0.05).In conclusion,dietary supplementation with yeast selenium alleviated endometrial inflammation of goats under high cortisol levels,and its regulatory mechanism may be related to MEK1/2-ERK1/2 pathway.关键词
MEK1/2-ERK1/2通路/酵母硒/皮质醇/山羊/大肠杆菌Key words
MEK1/2-ERK1/2 pathway/selenium yeast/cortisol/goat/Escherichia coli分类
农业科技引用本文复制引用
张敏,崔璐莹,袁长宁,郑方玲,李俊,郭龙,刘康军,董俊升,王亨,李建基..MEK1/2-ERK1/2通路参与硒在高皮质醇水平下对山羊子宫内膜炎症反应的抑制作用[J].中国兽医科学,2024,54(2):267-275,9.基金项目
国家自然科学基金项目(32072937,31802253,32102735) （32072937,31802253,32102735）
江苏现代农业产业技术体系建设专项(JATS[2023]499) （JATS[2023]499）
江苏省基础研究计划青年基金项目(BK20210808) （BK20210808）
江苏省333高层次人才培养工程资助项目 （）
江苏省(扬州大学)研究生科研与实践创新计划项目(KYCX22_3555,SJCX21_1642) （扬州大学）
高等学校学科创新引智计划项目(D18007) （D18007）
江苏高校优势学科建设工程资助项目(PAPD) （PAPD）
