中国生物防治学报2024，Vol.40Issue(1)：52-60,9.DOI:10.16409/j.cnki.2095-039x.2023.01.031
冻土毛霉菌株BO-1对韭菜迟眼蕈蚊的致病力及防治应用评价
Pathogenicity and field control efficiency of Mucor hiemalis BO-1 against Bradysia odoriphaga Larvae
摘要
Abstract
Mucor hiemalis BO-1 is a newly reported entomopathogenic fungus that infects Bradysia odoriphaga,a destructive root maggot.However,the pathogenicity and field control efficiency of M.hiemalis BO-1 against B.odoriphaga are unknown.In this study,we analyzed the pathogenicity of M.hiemalis BO-1 to B.odoriphaga larvae and its relation to temperature and evaluated its application value by pot and field experiments.The results indicated that M.hiemalis BO-1 was of acute pathogenicity against B.odoriphaga larvae and was as toxic as some chemical pesticides.At 3 d after treatment,the median lethal concentrations(LC50)to the 2nd and 4th instar larvae were 3.714× 105 spores/mL and 4.680 × 106 spores/mL,respectively.Moreover,M.hiemalis BO-1 also showed pathogenic activities against B.difformis and Delia antiqua larvae.The optimal temperatures for high pathogenic activity of M.hiemalis BO-1 were 16-28 ℃,especially at 20-24 ℃.The results of pot and field experiments confirmed excellent control efficiency of M.hiemalis BO-1 against B.odoriphaga larvae,which exceeded 80％at 7 d after treatment with spore concentration of 1 X 107 spores/mL,the equivalence to the chemical insecticide clothianidin.In addition,M.hiemalis BO-1 exhibited high persistence in control of B.odoriphaga.Therefore,M.hiemalis BO-1 is of high perspective as a biocontrol agent against B.odoriphaga.关键词
韭菜迟眼蕈蚊/冻土毛霉/昆虫病原真菌/致病力/防治效果Key words
Bradysia odoriphaga/Mucor hiemalis/entomopathogenic fungi/pathogenicity bioassay/control efficiency分类
农业科技引用本文复制引用
祝国栋,丁文娟,邱季雨,王燕,薛明,赵海朋,张国福..冻土毛霉菌株BO-1对韭菜迟眼蕈蚊的致病力及防治应用评价[J].中国生物防治学报,2024,40(1):52-60,9.基金项目
山东省自然科学基金(ZR201911130528) （ZR201911130528）
聊城大学博士启动基金(318051815) （318051815）
国家级大学生创新创业项目(CXCY2022042) （CXCY2022042）
