中国生物防治学报2024，Vol.40Issue(1)：61-70,10.DOI:10.16409/j.cnki.2095-039x.2023.11.005
金龟子绿僵菌fluG基因的敲除及对产孢的影响
Effects of fluG Gene Knockout on Sporulation in Metarhizium anisopliae
摘要
Abstract
The fungal genefluG is involved in the regulation of conidial generation.However,the role offluG in the entomopathogenic fungus Metarhizium anisopliae is rarely reported.To investigate the role offluG in M.anisopliae,we constructed a mutant strain of M.anisopliae with fluG knocked out by DNA homologous recombination to analyze its effect on the sporulation characteristics.The target vector pDHt/sk-fluG-Ben was constructed by using benomyl resistance gene ben as selective marker and sequence offluG gene flanks as homologous arms.The target vector was transformed into the protoplasts of M.anisopliae by PEG mediating,and the resistant transformants of benomyl were obtained.According to the test of target genes and marker genes,the mutant strains of M.anisopliae with fluG knocked out were determined.Phenotypic analysis showed that the fluG mutant still maintained benomyl resistance after 5 generations of subculture.The mutant colonies looked loose and flocculent,grew significantly slower than the wild type,and could not or only produce a very small number of conidia.These results indicate that the knockout of fluG gene affect the development of the strain and prevent conidia formation,showing fluG an important gene in the regulation of spore-producing of M.anisopliae.This study establishes the basis for further elaborating the regulation mechanism of M.anisopliae conidiation.关键词
基因敲除/fluG基因/产孢调控/同源重组/昆虫病原真菌Key words
gene knockout/fluG gene/sporulation regulation/homologous recombination/entomopathogenic fungus分类
农业科技引用本文复制引用
王苗苗,王广君,农向群,蔡霓,刘蓉,宋红岩,涂雄兵,张泽华..金龟子绿僵菌fluG基因的敲除及对产孢的影响[J].中国生物防治学报,2024,40(1):61-70,10.基金项目
国家重点研发计划(2022YFD1400505,2021YFD1000500,2017YFD0201205) （2022YFD1400505,2021YFD1000500,2017YFD0201205）
中央级公益性科研院所基本科研业务费专项(S2022XM11,Y2022LM31) （S2022XM11,Y2022LM31）
