中华神经创伤外科电子杂志2023，Vol.9Issue(6)：325-332,8.DOI:10.3877/cma.j.issn.2095-9141.2023.06.002
改良大鼠挫伤型脊髓损伤模型的制备与评估
Preparation and evaluation of an improved model of contusion spinal cord injury in rats
摘要
Abstract
Objective To prepare a rat contusion SCI model using a novel spinal cord injury(SCI)impactor and evaluate the model,in order to lay the foundation for exploring the mechanism of neuropathic pain after SCI and early intervention and treatment.Methods One hundred adult male SD rats were randomly divided into Control group(n=20),Sham group(n=40)and SCI group(n=40).Rats in SCI group were exposed to T10 segments and the SCI model was established by modified Allen's method.Rats in Sham group were exposed to T10 segments without SCI,while rats in Control group were not intervened.Open field test(BBB score)was used to evaluate the motor function of rats 1 d before injury and 1,3,7,14,21 and 28 d after injury.The mechanical foot retraction threshold(MWT)of the hind limb was measured 1 d before injury and 14,21 and 28 d after injury,and the histopathological changes of the spinal cord were detected by hematoxylin eosin(HE)staining to evaluate whether the SCI model was successfully constructed.Results(1)The BBB scores of three groups of rats at 1,3,7,14,21,and 28 d after injury were compared,and the differences were statistically significant(P＜0.05).Among them,the BBB scores of the SCI group were lower than those of the Control group and the Sham group,and the differences were statistically significant(P＜0.05).The BBB scores of the SCI group were all 0 points on the 1st day after injury,and showed an upward trend at 3,7,14,21,and 28 d after injury,and the difference between any two time points in pairwise comparison was statistically significant(P＜0.05).(2)There was no statistically significant difference in rat hindlimb MWT among the three groups 1 d before injury(P＞0.05),but there was a statistically significant difference at 14,21,and 28 d after injury(P＜0.05).Moreover,the rat hindlimb MWT of SCI group was significantly lower than that of the Control group and Sham group,and the difference was statistically significant(P＜0.05).The rat hindlimb MWT of SCI group rats showed a trend of first decreasing and then rebounding at 1 d before injury and 14,21,and 28 d after injury.Except for no statistically significant difference between 21 d after injury and 28 d after injury(P＞0.05),there were statistically significant differences in hindlimb MWT at all other time points(P＜0.05).(3)On the 14 d after injury,HE staining showed extensive infiltration of spinal cord glial cells.A large number of glial cells differentiated and proliferated 21 d after injury,and a large amount of glial fibers were generated 28 d after injury.Conclusion The contusion SCI model established by using the new percussion device has the advantages of digitalization,stability,standardization,strong operability and good repeatability,can provide a standardized model for the pathological mechanism of neuropathic pain formation after SCI and the study of targeted drugs.关键词
挫伤型脊髓损伤/改良动物模型/神经病理性疼痛/机械缩足阈值Key words
Contusion spinal cord injury/Improved animal model/Neuropathic pain/Mechanical withdrawal threshold引用本文复制引用
陈业煌,陈恺钦,薛亮,吴箭午,黄预备,魏梁锋,曾炳香,王守森..改良大鼠挫伤型脊髓损伤模型的制备与评估[J].中华神经创伤外科电子杂志,2023,9(6):325-332,8.基金项目
Natural Science Foundation of Fujian Province(2021J011276) （2021J011276）
The Project of The 900th Hospital of Joint Logistic Team(2020L18) （2020L18）
The Prefect of Joint Logistics Key Specialized Medicine(LQZD-SW)福建省自然科学基金(2021J011276) （LQZD-SW）
联勤保障部队第九○○医院临床应用研究专项(2020L18) （2020L18）
联勤医学重点专科项目(LQZD-SW) （LQZD-SW）
