中华神经创伤外科电子杂志2023，Vol.9Issue(6)：333-337,5.DOI:10.3877/cma.j.issn.2095-9141.2023.06.003
舒巴坦对局灶性脑缺血再灌注大鼠脑组织损伤的影响
Effect of sulbactam on brain tissue injury in rats with focal cerebral ischemia-reperfusion
摘要
Abstract
Objective To explore the efect of sulbactam on brain tissue injury in rats with focal cerebral ischemia-reperfusion.Methods Thirty male SD rats were selected and randomly divided into a sham group,a model group and a sulbactam group,with 10 rats in each group.The right cerebral ischemia-reperfusion model was prepared by thread embolism method.Thirty minutes after cerebral ischemia,the model group was intraperitoneally injected with 10 μL normal saline,the sulbactam group was intraperitoneally injected with 10 µL sulbactam,and the sham group was not given any intervention.Longa score was used to evaluate the neurological function of rats,Image-ProExpress imaging was used to analyze cerebral infarction volume,and immunohistochemical staining was used to detect serum glial glutamate transporter-1(GLT-1)levels.Thirty days after surgery,Morris water maze was used to test learning and memory function,and the escape latency on days 1,2,3,4,and 5 was compared.Results The neurological function score and cerebral infarction volume in the sham group were 0,while those in the sulbactam group were significantly lower than those in the model group(P＜0.05).The serum GLT-1 levels of the three groups were statistically significant(P＜0.05),the serum GLT-1 levels of the sulbactam group and the model group were significantly higher than those of the sham group,while the sulbactam group was significantly lower than the model group(P＜0.05).On the first day of test,the escape latency of the three groups was not statistically significant(P＞0.05).On the 2nd to 5th day of testing,the escape latency of the three groups was statistically significant(P＜0.05),the escape latency of the sulbactam group and the model group was significantly higher than that of the sham group(P＜0.05),and the escape latency of the sulbactam group was significantly lower than that of the model group(P＜0.05).Conclusion Sulbactam can effectively improve nerve function,learning and memory function,reduce cerebral infarction volume and serum GLT-1 level in rats with focal cerebral ischemia-reperfusion.关键词
舒巴坦/局灶性脑缺血/缺血再灌注/神经功能/脑梗死容积/学习记忆功能/胶质细胞谷氨酸转运体-1Key words
Sulbactam/Focal cerebral ischemia/Ischemia reperfusion/Neurological function/Cerebral infarction volume/Learning and memory function/Glial glutamate transporter-1引用本文复制引用
崔鑫,张鹏,古同男,赵艳芝..舒巴坦对局灶性脑缺血再灌注大鼠脑组织损伤的影响[J].中华神经创伤外科电子杂志,2023,9(6):333-337,5.基金项目
Scientific Research Training Fund of Yanjing Medical College in 2020(20kyqd02) （20kyqd02）
Research Project of China Association of Ethnic Medicine(2022Z1041-570221)燕京医学院2020年科研培育基金(20kyqd02) （2022Z1041-570221）
中国民族医药学会科研项目(2022Z1041-570221) （2022Z1041-570221）
