转化医学杂志2023，Vol.12Issue(6)：314-318,356,6.DOI:10.3969/j.issn.2095-3097.2023.06.004
粒细胞集落刺激因子联合羟基脲对慢性粒细胞白血病患者疗效及Bcl-2、Bax、Smoothened蛋白表达的影响
Effect of Granulocyte Colony-stimulating Factor Combined with Hydroxyurea in Patients with Chronic Myelogenous Leukemia and Its Effect on Bcl-2,Bax and Smoothened Protein Expression
摘要
Abstract
Objective To investigate the effect of granulocyte colony-stimulating factor(G-CSF)combined with Hydroxyurea in patients with chronic myelogenous leukemia(CML)and its impact on Bcl-2,Bax and Smoothened protein ex-pression.Methods A total of 100 patients with CML admitted from April 2018 to April 2020 were selected and divided into G-CSF group(n = 50)and combination treatment group(n = 50)according to the random number table method.In the G-CSF group,recombinant human granulocyte stimulating factor injection was given,and in the combination treatment group,recombinant human granulocyte stimulating factor injection combined with Hydroxyurea was given.Platelet parameters,white blood count(WBC),serum-related factors[interleukin-10(IL-10),tumor necrosis factor-α(TNF-α),interleukin-8(IL-8),cyclooxygenase-2(COX-2),transforming growth factor-β1(TGF-β1)],and Smoothened protein expression in Bcl-2,Bax and CD34 + cells before and after treatment were compared between the two groups.The clinical efficacy and the occurrence of adverse reactions were compared between the two groups.Results After treatment,the platelet count and ser-um TGFβ1 level in the combination treatment group were higher than those in the G-CSF group,while the platelet distribution width,mean platelet volume,WBC and serum levels of IL-10,TNF-α,IL-8 and COX-2 were lower(P<0.05).After treat-ment,the Bcl-2 and Smoothened protein expression were lower,while the expression of Bax protein was higher in combination treatment group than in G-CSF group(P<0.05).Compared with G-CSF group,the total effective rate of the combination treatment group was higher(P<0.05).There was no significant difference in the incidence of adverse reactions between the two groups(P>0.05).Conclusion G-CSF combined with Hydroxyurea in the treatment of CML can regulate the hemato-poietic function and immune function of patients,reduce the inflammatory response and WBC level,and effectively inhibit the symptoms of leukemia cell proliferation,thus improving the condition.关键词
慢性粒细胞白血病/粒细胞集落刺激因子/羟基脲/环氧合酶-2/转化生长因子-β1/白细胞计数/血小板计数/SmoothenedKey words
Chronic myeloid leukemia/Granulocyte colony-stimulating factor/Hydroxyurea/Cyclooxygenase-2/Transforming growth factor-β1/White blood cell count/Platelet count/Smoothened分类
医药卫生引用本文复制引用
李彦卿,许晓炜,曹宇峰,刘江,王磊,王莉丽..粒细胞集落刺激因子联合羟基脲对慢性粒细胞白血病患者疗效及Bcl-2、Bax、Smoothened蛋白表达的影响[J].转化医学杂志,2023,12(6):314-318,356,6.基金项目
河北省2022年度医学科学研究课题计划项目(20221900) （20221900）
张家口市科学技术研究与发展计划项目(1821112D) （1821112D）
