中药材2023，Vol.46Issue(7)：1608-1612,5.DOI:10.13863/j.issn1001-4454.2023.07.004
半夏枯斑病病原鉴定
Identification of the Pathogen of Pinellia ternata Blight
摘要
Abstract
Objective:To identify the species and taxonomic status of pathogenic bacteria of Pinellia ternata blight.Methods:Patho-genic bacteria were isolated and identified by tissue separation method,the pathogenicity of pathogenic bacteria was verified by Koch's rule,and the taxonomic status of pathogenic bacteria was determined by morphological identification combined with multi-gene phyloge-netic analysis.Results:Five strains were isolated from the disease plant of Pinellia ternata by tissue separation,and BXHM-1 and BX-HM-2 were proved to cause Pinellia ternata leaf disease according to Koch's rule.Based on the multi-gene phylogenetic analysis of ITS,Tub-2 and TEF1-α gene sequences,strains BXHM-1 and BXHM-2 clustered on the same evolutionary cladism as the reported potato gangrene bacterium Boeremia exigua.Based on the morphological and molecular identification results,the blight pathogen of Pinellia ter-nata in Hezhang county,was identified as Boeremia exigua Guizhou province.Conclusion:The Pinellia ternata leaf disease caused by Boeremia exigua is reported for the first time.This study provides basic research for the prevention and control of this disease.关键词
半夏枯斑病/马铃薯坏疽菌/分子鉴定Key words
Blight of Pinellia ternata(Thunb.)Breit./Boeremia exigua/Molecular identification分类
植物保护学引用本文复制引用
何海永,李继业,谭清群,赵玳琳..半夏枯斑病病原鉴定[J].中药材,2023,46(7):1608-1612,5.基金项目
植物病虫害生物学国家重点实验室贵州研究中心创新能力提升项目(黔科合服企[2020]4007) （黔科合服企[2020]4007）
贵州省科技支撑计划项目(黔科合支撑[2019]2315号 （）
科合支撑[2020]4Y114号) （）
