中药材2023，Vol.46Issue(10)：2425-2429,5.DOI:10.13863/j.issn1001-4454.2023.10.009
酸枣选育新品种遗传多样性的SCoT标记分析
SCoT Lakel Analysis on Genetic Diversity of New Cultivars of Ziziphus jujuba var. spinosa
摘要
Abstract
Objective:To analyze the genetic diversity and genetic relationship of new varieties and cultivated varieties of Ziziphus jujuba var.spinosa germplasm by SCoT molecular marker technology,so as to lay a solid research foundation for the protection,improve-ment and optimization of the germdasm resources of Ziziphus jujuba var.spinosa.Methods:The primers with good band repeatability and high polymorphism were screened from 100 SCoT primers for PCR amplification of the test materials,and the genetic similarity coeffi-cient between varieties was calculated.The cluster analysis was performed by unweighted average distance method(UPGMA).Results:17 primers were screened out from 100 SCoT primers,a total of 210 fragments were amplified,and each primer produced an average of 12.35 fragments.There were 187 polymorphic bands,and the polymorphism ratio was 89.05％.The genetic similarity coefficients of 22 Ziziphus jujuba var.spinosa germplasms were 0.561 to 0.941.The cluster analysis results showed that at a genetic similarity coefficient of 0.72,22 germplasms were clustered into three groups.Conclusion:This study provides a theoretical basis for identification and protec-tion of germplasm resources,molecular assisted breeding and exploitation of Ziziphus jujuba var.spinosa germplasm resources.关键词
酸枣/新品种/亲缘关系/遗传多样性/SCoT分子标记Key words
Ziziphus jujuba var.spinosa(Bunge)Hu ex H.F.Chow./New varieties/Genetic relationship/Genetic diversity/SCoT molecular marker分类
中医学引用本文复制引用
王琳,赵红艳,王云达,杜宪臣,曹岩,郭庆梅..酸枣选育新品种遗传多样性的SCoT标记分析[J].中药材,2023,46(10):2425-2429,5.基金项目
山东省自然科学基金项目(ZR2021MH123) （ZR2021MH123）
济南市农业应用技术创新计划项目(CX202110) （CX202110）
山东省科技型中小企业创新能力提升工程项目(2022TSGC2181) （2022TSGC2181）
