中药材2023，Vol.46Issue(12)：3005-3009,5.DOI:10.13863/j.issn1001-4454.2023.12.016
鲜地黄酚酸类成分研究
Study on Phenolic Acids of Fresh Rehmannia glutinosa
摘要
Abstract
Objective:To study the phenolic acids from the fresh Rehmannia glutinosa and the biological activities.Methods:The compounds from the 70%acetone extract of fresh Rehmannia glutinosa were isolated and purified by silica gel,MCI gel CHP-20,ODS,Sephadex LH-20 and preparative HPLC.The structures were identified on the basis of MS,NMR and physicochemical properties.MTT method was used to evaluate the proliferation effect of the RPASMC cells and all isolated compounds were evaluated in vitro for their α-glucosidase inhibitory activity.Results:Sixteen compounds were isolated from the fresh Rehmannia glutinosa and identified as C-vera-troyl glycol(1),cinnamic acid(2),ferulaic acid(3),cis-ferulaic acid(4),caffeic acid(5),ω-hydroxypropioguaiacone(6),4-hydroxy-phenethyl alcohol(7),acteoside(8),clerodenoside A(9),benzoic acid(10),anisic acid(11),4-hydroxybenzoic acid(12),vanillic acid(13),vanillin(14),3,4-dihydroxybenzene formaldehyde(15),syringic acid(16),respectively.Conclusion:Among them,com-pounds 1,4,6,9,11,and 15 are isolated from Rehmannia genus for the first time.Compounds 1,15,and 16 markedly inhibite the prolif-eration of RPASMC cells without obvious toxicity and all compounds show weak α-glucosidase inhibitory activity.关键词
地黄/化学成分/酚酸类/RPASMC细胞增殖/α-葡萄糖苷酶抑制活性Key words
Rehmannia glutinosa Libosch./Chemical constituents/Phenolic acids/RPASMC cells proliferation/α-Glucosidase in-hibitory activity分类
中医学引用本文复制引用
陈旭,曹彦刚,张宇涵,刘晏灵,任英杰,郑晓珂,冯卫生..鲜地黄酚酸类成分研究[J].中药材,2023,46(12):3005-3009,5.基金项目
国家重点研发计划项目(2017YFC1702800) （2017YFC1702800）
河南省重大科技专项(171100310500) （171100310500）
河南省科技攻关项目(232102311214) （232102311214）
