中药材2023，Vol.46Issue(10)：2443-2450,8.DOI:10.13863/j.issn1001-4454.2023.10.012
金樱子蜜制前后UPLC指纹图谱与外观颜色的相关性研究
Study on the Correlation Between UPLC Fingerprint and Appearance Color of Rosae Laevigatae Fructus Before and After Honey Processing
摘要
Abstract
Objective:To study the differences and change laws of UPLC fingerprint and chromaticity values of Rosae Laevigatae Fructus before and after honey processing.Methods:The UPLC fingerprints and chromaticity values of the pulps of Rosae Laevigatae Fructus and honey-processed Rosae Laevigatae Fructus were determined by UPLC and spectrophotometer,the differences between the pulps of Rosae Laevigatae Fructus and honey-processed Rosae Laevigatae Fructus were analyzed according to paired sample t test and orthogonal partial least squares analysis(OPLS-DA).At the same time,principal component analysis(PCA)and Pearson correlation analysis were used to analyze the changes in the processing of honey Rosae Laevigatae Fructus and determine the processing end point.Results:There were obvious differences in UPLC fingerprints between the pulps of Rosae Laevigatae Fructus and honey-processed Rosae Laevigatae Fructus.15,18 common peaks were calibrated,respectively.Three components were identified,which were gallic acid,5-hydroxymethylfurfural and ellagic acid,among which 5-hydroxymethylfurfural was the newly added component after honey processing.The results of OPLS-DA showed that chromatographic peaks 2,3,7,9,16,18 were the main different components(VIP＞1)of pulps of Rosae Laevigatae Fructus and honey-processed Rosae Laevigatae Fructus,which were basically consistent with the results of paired sam-ple t test.The results of Pearson correlation analysis showed that the chromaticity values L*,b*,E* of different processing times were significantly or very significantly correlated with the unit peak area of common peaks 2 to 4,7,16 to 18 of the fingerprint(P＜0.05).Combined with the principal component analysis,the appropriate processing time of 14 to 26 min was determined.Conclusion:The estab-lished UPLC fingerprint and chroma determination method can be used for the identification of pulps of Rosae Laevigatae Fructus and honey-processed Rosae Laevigatae Fructus and the determination of the processing end point.关键词
金樱子肉/蜜金樱子肉/指纹图谱/色度值/多元统计分析Key words
Pulp of Rosae Laevigatae Fructus/Pulp of honey-processed Rosae Laevigatae Fructus/Fingerprint/Chromaticity val-ue/Multivariate statistical分类
中医学引用本文复制引用
邓怡芳,程钰洁,钟志奎,潘玲,梁丽金,邱彩月,黄瑶..金樱子蜜制前后UPLC指纹图谱与外观颜色的相关性研究[J].中药材,2023,46(10):2443-2450,8.基金项目
中华人民共和国工业和信息化部2022年产业技术基础公共服务平台(2022-230-221) （2022-230-221）
