中药材2023，Vol.46Issue(7)：1638-1642,5.DOI:10.13863/j.issn1001-4454.2023.07.009
家鸡GBE1基因克隆与生物信息学分析
Cloning and Bioinformatics Analysis of GBE1 Gene in Gallus gallus
摘要
Abstract
Objective:To clone 1,4-α-glucan branchase 1(GBE1)gene and analyze its encoding protein bioinformatics and differ-ential expression.Methods:The full-length cDNA of GBE1 was amplified by PCR based on the transcriptome data of Gallus gallus and analyzed by bioinformatics.qRT-PCR assay was used to detect gene expression differences in 6 tissues of domestic Gallus gallus.Re-sults:The full-length of GBE1 gene was 2 145 bp and the open reading frame(ORF)was 2 130 bp,encoding 709 amino acids.The rela-tive molecular weight of the GBE1 protein was 81.993,the theoretical isoelectric point was 6.25,the stability index was 35.40,and the average hydrophilic value was-0.484,which belonged to stable acidic hydrophilic protein.GBE1 protein contained 67 phosphorylation sites,and there was no transmembrane region and signal peptide sequence.It was mainly located in cytoplasm,nucleus,mitochondria and cellular fluid.The secondary structure mainly consisted of α-helix,β-folding,random curling and extension chains,and the tertiary structure showed that the protein was a monomer.The homology analysis showed that the amino acid sequence of GBE1 protein in do-mestic Gallus gallus was closely related to that of Numida meleagris.The qRT-PCR results showed that GBE1 gene was expressed in all the tissues of heart,liver,spleen,lung,kidney and endothelium corneum gigeriae galli,but the expression level in liver and endothelium corneum gigeriae galli was significantly higher than that in other organs.Conclusion:The results of this study lay a foundation for further research on the function of GBE1 gene and the regulation mechanism of dextran branch enzyme in Gallus gallus.关键词
家鸡/鸡内金/GBE1/基因克隆/生物信息学Key words
Gallus gallus domesticus Brisson/Endothelium corneum gigeriae galli/GBE1/Gene cloning/Bioinformatics分类
中医学引用本文复制引用
袁铭君,李军德,李颖,张恬..家鸡GBE1基因克隆与生物信息学分析[J].中药材,2023,46(7):1638-1642,5.基金项目
国家自然科学基金青年科学基金项目(82104346) （82104346）
中国中医科学院科技创新工程项目(CI2021A04012) （CI2021A04012）
中央级公益性科研院所基本科研业务费专项(ZZXT202007) （ZZXT202007）
