中药材2023，Vol.46Issue(7)：1655-1663,9.DOI:10.13863/j.issn1001-4454.2023.07.012
基于HS-GC-MS及网络药理学技术解析艾叶不同炮制方法减毒的物质基础及分子机制
Study on Material Basis and Molecular Mechanism of Attenuating Toxicity of Artemisiae Argyi Folium by Different Processing Methods Based on HS-GC-MS and Network Pharmacology
摘要
Abstract
Objective:To explore the material basis and molecular mechanism of attenuating toxicity in the processing of Artemisiae Argyi Folium based on the difference of volatile components in Artemisiae Argyi Folium and its stir-fry,vinegar samples.Methods:Head-space-gas chromatography-mass spectrometry(HS-GC-MS)was used to identify the volatile components in Artemisiae Argyi Folium and its products.Multivariate statistical methods were used to analyze the different components of raw and processed products,and to analyze the possible basis of attenuated substances.With the help of network pharmacology,the targets and pathways related to hepatotoxicity of different components were explored to explore the molecular mechanism of processing attenuated toxicities of Artemisiae Argyi Folium.Results:A total of 41 chemical components were identified.The contents of 17 different components decreased after stir-frying and 16 different components decreased after roasting with vinegar.The results of network pharmacology showed that 10 and 12 components of Artemisiae Argyi Folium acted on 137 and 50 intersection genes and regulated multiple signaling pathways to reduce liver toxicity.Con-clusion:This study preliminarily explains the effects of different processing methods of Artemisiae Argyi Folium on volatile components and the potential molecular mechanism of alleviating liver toxicity,which can provide experimental basis and data support for clarifying the scientific connotation of processing attenuated toxicities of Artemisiae Argyi Folium.关键词
艾叶/炮制减毒/HS-GC-MS/多元统计分析/肝毒性/网络药理学Key words
Artemisiae Argyi Folium/Attenuating toxicity by processing/HS-GC-MS/Multivariate statistical analysis/Hepatoto-xicity/Network pharmacology分类
中医学引用本文复制引用
郎士跃,耿雪,化敏,李念虹,高奇,王变利,周倩..基于HS-GC-MS及网络药理学技术解析艾叶不同炮制方法减毒的物质基础及分子机制[J].中药材,2023,46(7):1655-1663,9.基金项目
山东省中药材及饮片标准研究(2020-247,2020-248) （2020-247,2020-248）
