中药材2023，Vol.46Issue(7)：1708-1713,6.DOI:10.13863/j.issn1001-4454.2023.07.021
不同种源北沙参香豆素含量及关键酶基因表达量差异研究
Study on the Differences of Coumarin Content and Key Enzyme Gene Expression of Glehniae Radix from Different Provenances
摘要
Abstract
Objective:To explore the differences of coumarin content and IPT gene relative expression in Glehniae Radix,so as to provide theoretical basis for quality assessment and further selection and breeding of Glehniae Radix.Methods:HPLC method was used to determine the contents of 9 coumarins in Glehniae Radix.The relative expression of isopentenyltransferase gene(IPT)was detected by real-time quantitative PCR.Results:The contents of coumarin in Glehniae Radix cultivated in Laiyang,Chifeng and wild species in Baxiandao,Jinshitan and Haiyang were higher.The relative expression of IPT gene was higher in Glehniae Radix from Chifeng and Haiyang.IPT gene expression was significantly positive correlated with psoralen and isophellolactone contents(P<0.01).Principal com-ponent analysis showed that Glehniae Radix cultivated in Laiyang and Haiyang were the planting area of high quality Glehniae Radix.Conclusion:In this study,the differences of coumarin content and IPT gene expression in Glehniae Radix from 8 provenances and the correlation between them are investigated,which can provide reference for the selection of provenances with high application value,and contribute to the further development and utilization of Glehniae Radix.关键词
北沙参/HPLC/香豆素/异戊烯基转移酶基因Key words
Glehniae Radix/HPLC/Coumarins/Isoprenyltransferase gene分类
医药卫生引用本文复制引用
张晴,白惠茹,张鑫,周春霞,逄玉娟,卞福花,李丽霞..不同种源北沙参香豆素含量及关键酶基因表达量差异研究[J].中药材,2023,46(7):1708-1713,6.基金项目
山东省重点研发计划项目(2019GSF108220) （2019GSF108220）
山东省草本植物种质资源普查(鲁财预指[2021]1号) （鲁财预指[2021]1号）
