中药材2023，Vol.46Issue(7)：1753-1759,7.DOI:10.13863/j.issn1001-4454.2023.07.030
基于ATF2和JAK2/STAT3信号通路研究麦门冬汤合千金苇茎汤联合顺铂对Lewis肺癌小鼠的抑瘤作用
Effect of Maimendong Decoction and Qianjinweijing Decoction Combined with Cisplatin on Tumor Inhibition of Mice with Lewis Lung Cancer Based on ATF2 and JAK2/STAT3 Signaling Pathway
摘要
Abstract
Objective:To study the effect of Maimendong decoction and Qianjinweijing decoction combined with cisplatin on tumor inhibition and immune function of mice with Lewis lung cancer based on ATF2 and JAK2/STAT3 signaling pathway.Methods:Lewis lung cancer model of C57BL/6 mice was established,and normal saline,Maimendong decoction and Qianjinweijing decoction,cisplatin,Maimendong decoction and Qianjinweijing decoction with cisplatin were given for 21 days.After continuous administration,the tumor bodies were exfoliated,the tumor weight and inhibitory rate were analyzed,and the thymus and spleen indexes were calculated.HE stai-ning was used to observe the histological changes of the tumor.Flow cytometry was used to detect the distribution and proportion of CD4+T lymphocytes(CD4+)and CD8+T lymphocytes(CD8+).The contents of TNF-α,IL-10,IL-12 and IL-16 in serum were deter-mined by ELISA.The expressions of invasion and metastasis related proteins ATF2,MMP-2 and MMP-9 were detected by immunohisto-chemistry,and the expressions of JAK2/STAT3 signaling pathway related proteins in tumor tissues were detected by Western Blot.Results:Compared with model group,the tumor weight of all treatment groups was significantly decreased(P<0.01).Compared with model group,the indexes of thymus and spleen in cisplatin group were decreased,while the indexes of thymus and spleen in Maimen-dong decoction and Qianjinweijing decoction group and Maimendong decoction combined Qianjinweijing decoction with cisplatin group were significantly increased(P<0.01).The degree of necrosis of tumor tissue was increased and the degree of division and proliferation was reduced under light microscope in each drug group.Compared with model group,the CD4+/CD8+ ratio of splenic lymphocyte sub-sets of mice in each drug group was significantly increased,and the contents of TNF-α,IL-10,IL-12 and IL-16 in serum and the protein expressions of ATF2,MMP-2,MMP-9,p-JAK2 and p-STAT3 in the tumor were significantly decreased(P<0.05 or P<0.01).Conclu-sion:Maimendong decoction and Qianjinweijing decoction with cisplatin alone or in combination have significant anti-cancer effects on Lewis lung cancer mice model,with little toxic side effects,and can enhance the body's immunity and reduce the level of inflammation.The mechanism may be related to the down-regulation of ATF2,MMP-2,MMP-9 expressions and the interference of JAK2/STAT3 signal transduction pathway.关键词
肺癌/麦门冬汤合千金苇茎汤/顺铂/激活转录因子2/Janus激酶2/转录激活因子3信号通路Key words
Lung cancer/Maimendong decoction and Qianjinweijing decoction/Cisplatin/Activated transcription factor 2/Janus kinase 2/signal transducer and activator of transcription 3 signaling pathway分类
医药卫生引用本文复制引用
孙莎莎,谢美萍,孙玉侠,李文婷,吴勉华,姜海英,姜泽群..基于ATF2和JAK2/STAT3信号通路研究麦门冬汤合千金苇茎汤联合顺铂对Lewis肺癌小鼠的抑瘤作用[J].中药材,2023,46(7):1753-1759,7.基金项目
国家自然科学基金项目(81603584,81804058) （81603584,81804058）
十四五江苏省重点学科-生物学资助项目(35091005006) （35091005006）
吴勉华全国名老中医药专家传承工作室(国中医药人教函[2022]75号) （国中医药人教函[2022]75号）
