组织工程与重建外科杂志2024，Vol.20Issue(1)：51-57,7.DOI:10.3969/j.issn.1673-0364.2024.01.002
利用CO2点阵激光精准构建小鼠背侧皮肤深Ⅱ度烧伤模型
Construction of mice dorsal skin deep second-degree burn model by carbon dioxide fractional laser
摘要
Abstract
Objective To accurately control the intensity and area of burns by using fractional carbon dioxide laser,and to explore the optimal energy intensity to prepare a model of deep second-degree skin burns.Methods Firstly,two groups of burn models with different energy intensities were obtained by using 50 mJ and 80 mJ CO2 fractional lasers,and the skin changes on the surface of the burn site were observed after burn modeling,and photographs were taken at 0,3,7 and 14 days after modeling to observe the healing of burns and analyze the wound healing rate.Subsequently,HE staining,Masson staining,Col Ⅰ and Col Ⅲ immunohistochemical staining were performed on the burn tissues taken at different time points,and the results were analyzed.Results Histological staining of the materials at different time points showed that there were significant differences in the depth of laser perforation,hair follicle regeneration and scar formation between the two groups.The immunohistochemical staining results of Col Ⅰ and Col Ⅲ showed that there were also significant differences in collagen regeneration between the two groups.Comprehensive analysis showed that the damage caused by 80 mJ energy intensity to the skin tissue of mice was more consistent with the characteristics of the deep second-degree burn model.Conclusion In this study,a model of deep second-degree burns with obvious obstacles in skin tissue regeneration was successfully constructed on the back of mice by carbon dioxide fractional laser,and the degree of damage was evaluated in detail by histology,immunohistochemistry,etc.,and finally the energy intensity of 80 mJ was screened for the construction of a deep second-degree burn model,which is expected to provide a new and favorable tool for studying the pathophysiology of burns and the screening of drugs for skin tissue regeneration.关键词
深Ⅱ度烧伤/二氧化碳点阵激光/皮肤损伤Key words
Deep second-degree burn model/Carbon dioxide fractional laser/Skin injury分类
医药卫生引用本文复制引用
王湘莹,卢婷婷,李凯祥,曾俊文,王会景,王伟,邓丹,付炜..利用CO2点阵激光精准构建小鼠背侧皮肤深Ⅱ度烧伤模型[J].组织工程与重建外科杂志,2024,20(1):51-57,7.基金项目
上海交通大学"交大之星"计划医工交叉研究基金(YG2021GD04). （YG2021GD04）
