组织工程与重建外科杂志2024，Vol.20Issue(1)：58-63,6.DOI:10.3969/j.issn.1673-0364.2024.01.003
CX3CR1对创伤性骨髓炎大鼠骨骼肌微纤维、ERK/MAPK信号通路及炎症反应的影响
Effects of CX3CR1 on skeletal muscle microfibers,ERK/MAPK signaling pathway and inflammatory response in rats with traumatic osteomyelitis
摘要
Abstract
Objective To explore the effects of CX3CR1 on inflammatory response,skeletal muscle microfibers and ERK/MAPK signaling pathway in rats with traumatic osteomyelitis.Methods Thirty SPF SD male rats were selected and divided into healthy group,model group and CX3CR1 inhibition group according to random number table method,with an average of 10 in each group.Traumatic osteomyelitis model was established in model group and CX3CR1 inhibition group.The healthy group and model group were routinely injected with normal saline intraperitoneally every day,and the CX3CR1 intervention group was treated by injecting CX3CR1 neutralizing antibody into the residual cavity.Serum levels of inflammatory cytokines interleukin(IL-6,IL-10,IL-1β,TGF-β)were detected by ELISA,skeletal muscle microfibers were detected by X-ray Norden score,and pathological changes were observed by HE staining.Extracellular regulated protein kinase 1/2(ERK1/2),mitogen activated protein kinase(MAPK)protein and mRNA in femur were detected by Western blotting and PCR.Results Compared with the healthy group,the contents of TGF-β,IL-1β,IL-10,IL-6 and other inflammatory factors were increased in the model group(P＜0.05).Compared with model group,the content of inflammatory factors in CX3CR1 inhibitory group was decreased(P＜0.05).Compared with the healthy group,the X-ray Norden score of the model group was increased over time(P＜0.05).Compared with model group,X-ray Norden score of CX3CR1 inhibition group was decreased(P＜0.05).HE staining showed that the bone cortex in the healthy group was intact.In the model group,there was a large number of inflammatory cell infiltration,focal abscess,and area necrosis.In the CX3CR1 inhibitory group,the large cortical bone was significantly improved and the inflammatory response was reduced.Compared with healthy group,ERK1/2 and MAPK protein and mRNA in model group were increased(P＜0.05).Compared with model group,the protein and mRNA of ERK1/2 and MAPK in CX3CR1 inhibition group were decreased(P＜0.05).Conclusion Inhibition of CX3CR1 can improve the disease response in traumatic osteomyelitis rats,which may be related to decreased inflammatory response,ERK/MAPK and improved skeletal muscle microfibers.关键词
创伤性骨髓炎/炎症反应/骨骼肌微纤维/细胞外调节蛋白激酶1/2/丝裂原活化蛋白激酶Key words
Traumatic osteomyelitis/Inflammatory response/Skeletal muscle microfibers/Extracellular regulated protein kinase 1/2/Mitogen activated protein kinase分类
医药卫生引用本文复制引用
曹众,李春燕,鲁广生,周琦石..CX3CR1对创伤性骨髓炎大鼠骨骼肌微纤维、ERK/MAPK信号通路及炎症反应的影响[J].组织工程与重建外科杂志,2024,20(1):58-63,6.基金项目
国家自然科学基金(81674001). （81674001）
