组织工程与重建外科杂志2024，Vol.20Issue(1)：75-82,8.DOI:10.3969/j.issn.1673-0364.2024.01.006
肉芽肿性乳腺炎病灶组织差异表达蛋白质筛选及关键分子验证
Identification of differentially expressed proteins of granulomatous mastitis and key cytokine validation
摘要
Abstract
Objective To identify the differentially expressed proteins in patients of granulomatous lobular mastitis(GLM)and screen the biomarkers that related to GLM,then to verify some key molecule according to the results.Methods The lesion tissues and adjacent normal tissues following surgical removal of affected tissues were collected from 3 GLM patients.Then the quantitative proteomics TMT technology was used to detect and quantitatively analyze the differentially expressed proteins,and to conduct functional analysis.Uniprot database and proteome discoverer software were applied to analyze the bioinformatic.The concentrations of key cytokine were analyzed by human antibody array.Results In this study,1 405 differentially expressed proteins were identified including 1 106 upregulated proteins and 299 downregulated proteins in GLM.Gene ontology(GO)annotation and functional cluster analyses showed that these differentially expressed proteins mainly involved in cellular processes,metabolic processes,protein metabolism,biological regulation.KEGG pathway analysis showed these differentially expressed proteins were mainly enriched in complement activation,immune system,inflammatory response.Cytokine quantitative analysis confirmed the increased expression of IL-1β,MCP2 and IL-6 in GLM tissures.Conclusion Differentially expressed proteins in granulomatous mastitis are involved in related signaling pathways and may be key molecules in the development of granulomatous mastitis.Specific proteins are the potential biomarkers that deserve further exploration.关键词
肉芽肿性乳腺炎/生物标志物/串联质谱标签/蛋白质组学/抗体芯片技术Key words
Granulomatous mastitis/Bioinformatics/Tandem mass tag/Proteomics/Antibody array technology分类
临床医学引用本文复制引用
周悦,叶媚娜,代秋颖,王冰,程一凡,陈红风..肉芽肿性乳腺炎病灶组织差异表达蛋白质筛选及关键分子验证[J].组织工程与重建外科杂志,2024,20(1):75-82,8.基金项目
上海申康医院发展中心第二轮临床三年行动计划重大临床研究项目(SHDC2020CR2051B) （SHDC2020CR2051B）
闵行区卫健委"龙华医院-闵行"中医外科乳腺专科联盟项目(LM05) （LM05）
龙华医院"科技创新培育计划"(PY2022016). （PY2022016）
