中药材2023，Vol.46Issue(11)：2692-2697,6.DOI:10.13863/j.issn1001-4454.2023.11.009
基于DNA条形码及HPLC指纹图谱的竹茹鉴别研究
Identification of Bambusae Caulis in Taenias Based on DNA Barcoding and HPLC Fingerprint
摘要
Abstract
Objective:To identify the original plants of Bambusae Caulis in Taenias from different sources by using DNA barcode and HPLC fingerprinting technology.Methods:The psbA-trnH sequence was used as DNA barcode for molecular identification.HPLC fingerprint of Bambusae Caulis in Taenias was established,and chemical pattern recognition methods such as cluster analysis,principal component analysis and discriminant analysis were used for classification.Results:The 22 batches of Bambusae Caulis in Taenias were identified as 7 batches of Bambusa tuldoides,9 batches of Phyllostachys glauca and 6 batches of others.11 common peaks were obtained by HPLC fingerprints and the samples were grouped into 3 groups by chemical pattern recognition analysis,which basically realized the differentiation of samples from different origin.At the same time,6 compounds were identified for the characterization of different origi-nal Bambusae Caulis in Taenias.Conclusion:The results of this study establish a set of effective methods for species identification of Bambusae Caulis in Taenias,and have guiding significance for solving the problem of authenticity identification and quality evaluation of Bambusae Caulis in Taenias.关键词
竹茹/DNA条形码/HPLC指纹图谱/化学模式识别Key words
Bambusae Caulis in Taenias/DNA barcoding/HPLC fingerprint/Chemical pattern recognition分类
中医学引用本文复制引用
罗宇琴,黄上书,李国卫,潘礼业,陈向东,索彩仙,孙冬梅..基于DNA条形码及HPLC指纹图谱的竹茹鉴别研究[J].中药材,2023,46(11):2692-2697,6.基金项目
国家工业和信息化部2019 年产业技术基础公共服务平台项目(2019-00902-1-2) （2019-00902-1-2）
