中药材2023，Vol.46Issue(6)：1342-1347,6.DOI:10.13863/j.issn1001-4454.2023.06.003
瑶药穿心藤组培快繁体系的建立
Establishment of Tissue Culture and Rapid Propagation System of Yao Medicine Amydrium hainanense
摘要
Abstract
Objective:To establish an efficient tissue culture and regeneration system for the preservation and utilization of the germplasm resources of Yao medicine Amydrium hainanense.Methods:Using the stem segment of Amydrium hainanense in the current year as explants,the effects of different base medium on adventitious bud induction,plant growth regulator ratio of different concentration on adventitious bud induction and differentiation,bud proliferation and rooting were studied,and the optimal medium for different culture stages was screened.Results:The optimal medium for adventitive bud induction of Amydrium hainanense was MS,6-BA 2.00 mg/L and NAA 0.20 mg/L,the induction rate was 94.63％.The optimal medium for adventitious bud proliferation was MS,6-BA 3.00 mg/L and NAA 0.40 mg/L,the proliferation coefficient was up to 10.30,the buds were green and robust.The optimal medium for rooting of tissue culture seedlings was MS and NAA 0.50 mg/L,the average number of rooting per plant was 6,and the rooting rate was 97.13％.Trans-planting seedlings after 6 days of cultivation grew healthily and the survival rate was the highest,reaching 100％.Conclusion:The tissue culture and regeneration system of Amydrium hainanense is established,which can lay a foundation for the protection of germplasm re-sources and industrial planting of Amydrium hainanense.关键词
穿心藤/茎段/组织培养/植株再生/药用植物Key words
Amydrium hainanense H.Li/Stem segment/Tissue culture/Plant regeneration/Medicinal plants分类
中医学引用本文复制引用
张雯,陈国华,苏韬,宋希强,孟新亚,陈红锋..瑶药穿心藤组培快繁体系的建立[J].中药材,2023,46(6):1342-1347,6.基金项目
广东省重点领域研发计划项目(2020B1111530004) （2020B1111530004）
中国科学院植物资源保护与可持续利用重点实验室开放基金项目(Y821171001) （Y821171001）
