中药材2023，Vol.46Issue(6)：1348-1352,5.DOI:10.13863/j.issn1001-4454.2023.06.004
黄芪冠腐病病原鉴定
Identification of the Pathogen of Astragalus membranaceus var.mongholicus Crown Rot
摘要
Abstract
Objective:To discover a new root disease of Astragalus membranaceus var.mongholicus root.The root crown epidermis was brown to black and was cracked,named Astragalus membranaceus var.mongholicus crown rot,so as to clarify the pathogenic species of the disease and provide theoretical basis for field control.Methods:The typical strains of the disease were isolated and cultured by tis-sue separation and identified by morphological and molecular biological methods.Results:Based on morphological identification,52 strains were isolated and divided into three types.The sequences of rDNA-ITS,TEF1-α,β-TUB genes were amplified and sequenced in representative strains,and the multigene association phylogeny was analyzed.Strain HJ108 was clustered with Fusarium equiseti.Strain HJ201 was clustered with Fusarium acuminatum.Strain HJ305 was clustered with Fusarium oxysporum.Conclusion:The pathogens cau-sing this disease are identified as Fusarium equiseti,Fusarium acuminatum and Fusarium oxysporum.The isolation frequency of Fusari-um equiseti is 57.7％,which is the dominant pathogen.关键词
黄芪/冠腐病/病原鉴定/致病性测定Key words
Astragalus membranaceus(Fisch.)Bge.var.mongholicus(Bge.)Hsiao/Crown rot/Pathogen identification/Pathogenic-ity determination分类
植物保护学引用本文复制引用
胡耀龙,高龙,申昊,乔凯,贾龙,李文强,任斌..黄芪冠腐病病原鉴定[J].中药材,2023,46(6):1348-1352,5.基金项目
宁夏回族自治区重点研发计划重点项目(2019BBF02029) （2019BBF02029）
