中药材2023，Vol.46Issue(9)：2178-2184,7.DOI:10.13863/j.issn1001-4454.2023.09.012
续断盐炙前后指纹图谱及成分含量差异研究
Study on Fingerprint and Component Content Differences of Dipsaci Radix Before and After Salt Frying
摘要
Abstract
Objective:To establish the fingerprint of Dipsaci Radix before and after salt frying,to simultaneously determine the con-tent of 6 components and comprehensively evaluate the quality of Dipsaci Radix before and after salt frying combined with chemomet-rics.Methods:HPLC method was used to establish the fingerprints of Dipsaci Radix and salt-processed Dipsaci Radix.The common peaks were identified by reference materials and the contents were determined.The fingerprint data before and after salt processing was analyzed by principal component analysis(PCA)and orthogonal partial least squares discriminant analysis(OPLS-DA).Results:Twen-ty-six and twenty-seven common peaks were identified by the fingerprints of Dipsaci Radix and salt-processed Dipsaci Radix,the simi-larity was 0.988～1.000,0.987～1.000,respectively.Six of the peaks were identified,which were loganic acid,chlorogenic acid,loga-in,isochlorogenic acid A,isochlorogenic acid C,asperosaponin Ⅵ.Nine different components were screened by PCA and OPLS-DA be-fore and after salt frying.The linear relationships of the six identified components were good within the concentration ranges.The average recoveries was 96.64%to 100.74%,RSD was 1.04%to 2.01%.The results of content determination showed that the contents of above six components in Dipsaci Radix and salt-processed Dipsaci Radix were 1.65%to 2.39%,0.70%to 0.89%,0.88%to 1.10%,0.93%to 1.16%,0.37%to 0.46%,4.30%to 6.39%and 2.06%to 2.45%,0.66%to 0.85%,0.99%to 1.23%,0.77%to 1.11%,0.37%to 0.53%,3.80%to 5.36%,respectively.Conclusion:This study established the fingerprint and content determination method of 6 components before and after Dipsaci Radix salt processing can provide reference for processing and quality control of Dipsaci Radix.关键词
续断/盐炙/指纹图谱/PCA/OPLS-DA/多成分含量测定Key words
Dipsaci Radix/Salt processing/Fingerprint/PCA/OPLS-DA/Multi-component content determination分类
医药卫生引用本文复制引用
唐晓琴,张丽艳,马四补,赵泽林,董贺,孙恒灿,王羿..续断盐炙前后指纹图谱及成分含量差异研究[J].中药材,2023,46(9):2178-2184,7.基金项目
贵州省科技计划项目(黔科合支撑[2023]一般395) （黔科合支撑[2023]一般395）
国家重点研发计划项目(2019YFC1712500) （2019YFC1712500）
贵州省科技计划项目(黔科合后补助[2020]3003) （黔科合后补助[2020]3003）
