中药材2023，Vol.46Issue(9)：2201-2205,5.DOI:10.13863/j.issn1001-4454.2023.09.016
贯叶连翘的化学成分及降糖活性研究
Study on the Chemical Constituents of Hypericum perforatum and Hypoglycemic Activity
摘要
Abstract
Objective:To study the chemical constituents of Hypericum perforatum and hyperglycemic activity.Methods:The 95%ethanol extract of the aerial part of Hypericum perforatum was isolated and purified by silica gel column chromatography,ODS column chromatography,Sephadex LH-20 column chromatography and preparative HPLC,the structures were identified by HR-ESI-MS and NMR spectral techniques.The α-glucosidase inhibitory assay(in vitro)was used to determine the hypoglycemic activity of the com-pounds.Results:Fourteen compounds were isolated from Hypericum perforatum and identified as pteryxin(1),3′,4′-disenecioyl-cis-khellactone(2),5,4′-dihydroxy-6,8-di-C-methyl-7-methoxy-flavanone(3),5,4′-dihydroxy-6-C-methyl-7-methoxy-flavanone(4),5,7-dihydroxy-3-methylchromone(5),5,7-dihydroxy-3-ethylchromone(6),ethyl-2,4,6-trihydroxybenzoate(7),vanillin(8),7-hydroxy-5-methoxy-4,6-dimethylphthalidc(9),α-tocopherol quinone(10),stigmasterol(11),acetyl-11-keto-β-boswellic acid(12),ferulic acid hexacosanyl ester(13),cosmosoic acid(14),respectively.Conclusion:Among them,compounds 1 to 4,10,12 to 14 are isolated from Hypericum genus for the first time,compounds 5 to 7,9 are isolated from Hypericum perforatum for the first time.Compounds 3,4 show better α-glucosidase inhibitory activity with IC50 values of 490.3,549.9 μmol/L,respectively,which are similar to that of acarbose with IC50 value of 500.1 μmol/L.关键词
贯叶连翘/二氢黄酮/香豆素/α-葡萄糖苷酶Key words
Hypericum perforatum L./Dihydroflavone/Coumarin/α-Glucosidase分类
医药卫生引用本文复制引用
李昕昕,赫军,殷伟峰,王利杰,陈欣,张维库,续洁琨..贯叶连翘的化学成分及降糖活性研究[J].中药材,2023,46(9):2201-2205,5.基金项目
中央高水平医院临床科研业务费(2023-NHLHCRF-CXYW-01,2022-NHLHCRF-YNZJ-01) （2023-NHLHCRF-CXYW-01,2022-NHLHCRF-YNZJ-01）
中日友好医院菁英计划(ZRJY2021-BJ06,ZRJY2021-TD06) （ZRJY2021-BJ06,ZRJY2021-TD06）
