中药材2023，Vol.46Issue(9)：2291-2295,5.DOI:10.13863/j.issn1001-4454.2023.09.031
丹酚酸B抑制HMGB1/TLR4/NF-κB通路改善顺铂诱导的急性肾损伤
Effect of Salvianolic Acid B on Cisplatin Induced Acute Kidney Injury in Mice by Inhibiting HMGB1/TLR4/NF-κB Pathway
摘要
Abstract
Objective:To investigate the effect of salvianolic acid B on cisplatin induced acute renal injury in mice and explore its mechanism.Methods:C57BL/6 mice were randomly divided into normal control group,model group and salvianolic acid B low(50 mg/kg),medium(100 mg/kg),high(200 mg/kg)dose groups.The mice in each group were given the corresponding dose of salvianolic acid B or normal saline once a day for a total of 5 days.On the third day,except for normal control group,mice in other groups were giv-en 20 mg/kg cisplatin intraperitoneally injected to establish mice acute kidney injury model,and the mice were killed 48 hours later.The serum and kidney tissues were collected,serum creatinine and urea nitrogen levels were detected,renal histopathological changes were observed by HE staining,apoptosis of cells in kidney tissues was evaluated by TUNEL staining,HMGB1 expression in kidney tis-sue was detected by immunohistochemistry,and Bcl-2,Bax,HMGB1,TLR4,p-NF-κB p65 protein expression in kidney tissue were de-tected by Western Blot.Results:Compared with the normal control group,the levels of serum creatinine and urea nitrogen in the model group were significantly increased,vacuole-like degeneration of renal tubule epithelial cells,more tubule types were seen in the lumen of the tubule,more positive cells were detected by TUNEL staining,and the ratio of Bax/Bcl-2 and the expressions of HMGB1,TLR4 and p-NF-κB p65 proteins in kidney tissues were significantly increased(P<0.05).Compared with model group,the levels of serum creati-nine and urea nitrogen in salvianolic acid B medium and high dose groups were significantly decreased,the tubular type of renal tubules was decreased,there were fewer TUNEL positive cells,the ratio of Bax/Bcl-2 and the expressions of HMGB1,TLR4 and p-NF-κB p65 protein were significantly decreased(P<0.05).Conclusion:Salvianolic acid B can alleviate cisplatin induced acute kidney injury in mice,and its mechanism may be related to inhibiting HMGB1/TLR4/NF-κB pathway.关键词
急性肾损伤/顺铂/丹酚酸B/HMGB1Key words
Acute kidney injury/Cisplatin/Salvianolic acid B/HMGB1分类
医药卫生引用本文复制引用
付宏鑫,殷思敏,官学苹,江嘉颖,周玖瑶,周园..丹酚酸B抑制HMGB1/TLR4/NF-κB通路改善顺铂诱导的急性肾损伤[J].中药材,2023,46(9):2291-2295,5.基金项目
国家自然科学基金项目(81803824,81673874) （81803824,81673874）
