植物研究2024，Vol.44Issue(2)：298-306,9.DOI:10.7525/j.issn.1673-5102.2024.02.015
羽衣甘蓝类受体激酶FERONIA基因克隆、表达及与相互作用蛋白分析
Gene Cloning,Expression and Interaction Protein Analysis of FERONIA in Brassica oleracea var.acephala
摘要
Abstract
FERONIA(FER)receptor kinase plays an important role in pollen and stigma recognition in flowering plants.To analyze the function of receptor-like kinase FERONIA(FER)in the pollination in ornamental kale(Brassica oleracea var.acephala),BoFER gene was cloned from the stigma of the self-incompatibility line(S13-bS13-b)of ornamental kale by RT-PCR,and the cloned cDNA sequence was 2 682 bp,which encoded 893 amino acids containing the highly conserved kinase domain and Ser/Thr kinase binding site,and the expression level of BoFER was analyzed by qRT-PCR.The results showed that the expression of BoFER in stigmas gradually increased after incompatible pollination,but gradually decreased after compatible pollination.Furthermore,the interaction between BoFER and known proteins related to self-incompatibility were analyzed by yeast two-hybrid.The results suggested that BoFER interacted with the kinase domain of S-locus receptor kinase BoSRK13-b.关键词
羽衣甘蓝/FERONIA/自交不亲和性/表达分析/酵母双杂交Key words
Brassica oleracea var.acephala/FERONIA/self-incompatibility/expression analysis/yeast two-hybrid分类
农业科技引用本文复制引用
荀宝茹,秦洪涛,马蕊,郭楠枫,刘运平,吴莹,蓝兴国..羽衣甘蓝类受体激酶FERONIA基因克隆、表达及与相互作用蛋白分析[J].植物研究,2024,44(2):298-306,9.基金项目
黑龙江省大学生创新创业训练计划项目(S202310225227) （S202310225227）
国家自然科学基金面上项目(31870300). （31870300）
