中药材2023，Vol.46Issue(6)：1376-1380,5.DOI:10.13863/j.issn1001-4454.2023.06.009
积雪草皂苷生物合成基因筛选与表达量分析
Screening and Expression Analysis of Saponins Biosynthesis Genes from Centella asiatica
摘要
Abstract
Objective:To screen the key enzyme genes related to the biosynthesis of Centella asiatica saponins and verify them by quantitative fluorescence PCR(qRT-PCR).Methods:The saponins content of Centella asiatica in different parts was determined by HPLC.The key enzyme genes related to Centella asiatica saponin biosynthesis were excavated by transcriptomics and KEGG enrichment analysis.qRT-PCR was used to verify the expression patterns of the extracted key enzyme genes in different tissues.Pearson method was used to analyze the correlation between key enzyme and saponins.Results:The saponins content in leaves was the highest among the five different tissues(root,stem,leaf,petiole and fruit).Four key enzyme genes related to the biosynthesis of Centella asiatica saponins were extracted and selected,which were oxidized squalene cyclase(CaOSC1,CaOSC2),squalene cyclase(CaSE)and squalene synthase(CaSS).The expression levels of these four key enzyme genes were the highest in leaves.Conclusion:The saponins content in different tissues of Centella asiatica is closely related to the expression levels of four key enzyme genes,which accumulate the necessary research basis for the future exploration of the regulation mechanism of the biosynthesis and accumulation of saponins in Centella asiatica.关键词
积雪草/皂苷/转录组/关键酶基因/表达模式Key words
Centella asiatica(L.)Urb./Saponins/Transcriptome/Key enzyme genes/Expression pattern分类
生物学引用本文复制引用
李红金,黄鼎,姚绍嫦,李良波,黄荣韶,谭勇,明如宏..积雪草皂苷生物合成基因筛选与表达量分析[J].中药材,2023,46(6):1376-1380,5.基金项目
广西科技基地和人才专项项目(桂科AD21220083) （桂科AD21220083）
广西中医药大学博士启动基金项目(2021BS010) （2021BS010）
广西中医药大学桂派中医药传承创新团队项目(2022B005) （2022B005）
民族药资源再生与生态保护适宜技术及产业化模式研究(2019YFC1712305) （2019YFC1712305）
