中药材2023，Vol.46Issue(11)：2754-2759,6.DOI:10.13863/j.issn1001-4454.2023.11.020
竹节参多糖的结构表征及体外免疫活性研究
Structural Characterization and Immune Activity In Vitro of Polysaccharides from Panax japonicus
摘要
Abstract
Objective:To isolate and purify the homogeneous polysaccharide from Panax japonicus,and to study its structure char-acterization and immunoactivity in vitro.Methods:The crude polysaccharides of Panax japonicus were prepared using water extraction and alcohol precipitation method.After deproteinization with Sevag method,the refined polysaccharides of Panax japonicus(PJPS)were obtained.The ethanol fractional precipitation method,DEAE-52 and Sephacryl S-400 column chromatography were used to isolate and purify homogeneous polysaccharide PPS.The relative molecular weight and monosaccharide composition of PPS were determined by high performance gel permeation chromatography(HPGFC-RID)and 1-phenyl-3-methyl-5-pyrazolone(PMP)pre-column derivatization HPLC,respectively.The methylation products were analyzed by GC-MS.IR,1 D-and 2 D-NMR analyses were used for structural charac-terization of PPS.The effects of PJPS and PPS on cell viability,release of NO,expression of tumor necrosis factor-α(TNF-α)and inter-leukin-6(IL-6)were observed using RAW 264.7 cell line of mouse mononuclear macrophages.Results:PPS was a glucan with a rela-tive molecular mass of 3.3 kDa.The backbone of PPS was α-D-(1→4)-connection of glucoside,with α-t-Glcp attached to the six posi-tion of each of the five Glcp sugar groups.PPS could promote RAW 264.7 cell proliferation.PJPS and PPS could promote the secretion of NO,TNF-α and IL-6.Conclusion:PPS is a homogeneous polysaccharide isolated from Panax japonicus with certain immunological ac-tivity.The study of the structural characterization and immune activity of PPS can provide a reference for further development and utili-zation of Panax japonicus.关键词
竹节参/多糖/结构表征/免疫活性Key words
Panax japonicus C.A.Mey./Polysaccharides/Structural characterization/Immune activity分类
医药卫生引用本文复制引用
董一鑫,陈洁,于萍,孟祎凡,苏比努尔·亚森,李航,邹海艳..竹节参多糖的结构表征及体外免疫活性研究[J].中药材,2023,46(11):2754-2759,6.基金项目
国家自然科学基金面上项目(81973564) （81973564）
北京市教委科技计划资助项目(KM201910025020) （KM201910025020）
