中药材2023，Vol.46Issue(11)：2767-2772,6.DOI:10.13863/j.issn1001-4454.2023.11.022
菝葜标准汤剂的HPLC特征图谱建立、化学计量学分析及含量测定
Establishment of Specific Chromatogram and Chemometric analysis,Content Determination of Smilacis Chinae Rhizoma Standard Decoction
摘要
Abstract
Objective:To establish a HPLC specific chromatogram and content determination method of Smilacis Chinae Rhizoma standard decoction.Methods:The HPLC specific chromatogram of 16 batches of Smilacis Chinae Rhizoma standard decoction was estael-ished,similarity evaluation,cluster analysis and principal component analysis were carried out,the contents of three components were determined,and the transfer rate and extraction rate were calculated.Results:The established HPLC specific chromatogram of 16 bat-ches of Smilacis Chinae Rhizoma standard decoction identified 14 common peaks,and 7 components were identified.The similarity be-tween the standard decoction samples and reference spectrum was 0.939 to 0.997.The results of cluster analysis and principal compo-nent analysis were basically the same,and the samples were divided into three categories.The extraction rates of 16 batches of Smilacis Chinae Rhizoma standard decoction were 9.14%to 13.60%,the contents of chlorogenic acid,astilobin,resveratrol were 9.98 to 21.91 mg/g,1.92 to 5.34 mg/g,0.10 to 0.93 mg/g,respectively.The transfer rates were 16.12%to 59.19%,6.69%to 33.70%,8.70%to 63.96%,respectively.Conclusion:The specific chromatogram and content determination method established in this study is stable and feasible,and can be used for quality evaluation of Smilacis Chinae Rhizoma standard decoction.关键词
菝葜/标准汤剂/HPLC/特征图谱/含量测定Key words
Smilacis Chinae Rhizoma/Standard decoction/HPLC/Specific chromatogram/Content determination分类
医药卫生引用本文复制引用
陈彦洁,罗鑫,蒋学青,谢谭芳,陈豪,王志萍..菝葜标准汤剂的HPLC特征图谱建立、化学计量学分析及含量测定[J].中药材,2023,46(11):2767-2772,6.基金项目
广西科技重大专项(桂科AA18126003) （桂科AA18126003）
