中药材2023，Vol.46Issue(10)：2382-2387,6.DOI:10.13863/j.issn1001-4454.2023.10.002
人参不定根诱导、增殖及皂苷积累的研究
Study on Induction,Proliferation and Saponin Accumulation of Adventitious Root of Panax ginseng
摘要
Abstract
Objective:To establish the induction system of Panax ginseng adventitious root and optimize the liquid culture and breeding conditions.Methods:The effects of auxin NAA and IBA on induction of Panax ginseng adventitious root were studied by or-thogonal experiment with MS as medium.The effects of different concentrations of NAA,IBA and their combinations on adventitious root growth and saponin content of Panax ginseng were investigated.Results:The seed embryo was the best type of explants to induce Panax ginseng adventitious root,and the best solid medium was MS with NAA 0.6 mg/L,IBA 1.0 mg/L,3％sucrose,0.8％agar,the induc-tion rate was 88.42％and induction time was 18 days.IBA was more beneficial to adventitious root growth than NAA in liquid culture.When NAA 2.0 mg/L,IBA 1.0 mg/L and NAA 1.0 mg/L with IBA 1.0 mg/L were added,the biomass and saponin content of adventi-tious root were higher,and the combination of NAA 1.0 mg/L and IBA 1.0 mg/L was the best for adventitious root proliferation.The optimal medium was MS with NAA 1.0 mg/L,IBA 1.0 mg/L,3％sucrose.Conclusion:The method of direct induction of adventitious root by different explants of Panax ginseng is established,and a large amount of adventitious root materials can be obtained in a short time,which provides a new drug source for the production of secondary metabolites of Panax ginseng.关键词
人参/不定根/诱导/增殖培养/人参皂苷Key words
Panax ginseng C.A.Mey./Adventitious root/Induction/Proliferation culture/Ginsenoside分类
医药卫生引用本文复制引用
梁佳,苗佳琪,郝甜甜,代明涛,丁万博,杨鹤,许永华..人参不定根诱导、增殖及皂苷积累的研究[J].中药材,2023,46(10):2382-2387,6.基金项目
吉林省科技发展计划项目(20220204079YY) （20220204079YY）
