中药材2023，Vol.46Issue(11)：2810-2817,8.DOI:10.13863/j.issn1001-4454.2023.11.030
白芍总苷调控AMPK/RANKL/OPG轴治疗CIA模型大鼠骨破坏的作用研究
Effect of Total Glucoside of Paeoniae Radix Alba on Bone Destruction in CIA Model Rats by Regulating AMPK/RANKL/OPG Axis
摘要
Abstract
Objective:To explore the effect of total glucoside of Paeoniae Radix Alba on bone destruction in CIA model rats by reg-ulating AMPK/RANKL/OPG axis.Methods:Sixty successful female Wistar rats were randomly divided into model group,AICAR group,leflunomide group,high,medium and low dose of total glucoside of Paeoniae Radix Alba groups with 10 in each group.Another 10 healthy female Wistar rats were selected as normal control group.After 4 weeks of administration,the changes of body mass and toe swelling were monitored,The histopathological changes of knee sunovium were observed by HE staining.Serum TNF-α,IL-1a,IL-6 and IL-10 levels were detected by ELISA.The mRNA and/or protein expressions of AMPK,OPG,RANKL and TRAP were detected by PCR and Western Blot.The destruction of articular cartilage and subchondral bone in rats was observed by color B-ultrasound and Micro-CT.Results:Compared with the model group,total glucoside of Paeoniae Radix Alba effectively alleviated joint swelling.In a dose-dependent manner,total glucoside of Paeoniae Radix Alba decreased the levels of TNF-α,IL-1a and IL-6 in serum and the expressions of synovial RANKL and TRAP mRNA and proteins in CIA model rats,and the expressions of OPG,p-AMPK mRNA and/or proteins in synovial membrane was increased(P<0.05 to P<0.001),and the effect of medium dose was similar to that of AICAR.Leflunomide did not sig-nificantly increase the mRNA expression of AMPK signal.Conclusion:The total glucosides of Paeoniae Radix Alba can ameliorate joint destruction in CIA rats to a certain extent,which may play the bone protective role of RA by regulating AMPK/RANKL/OPG signaling pathway.关键词
类风湿关节炎/AMPK/RANKL/OPG轴/白芍总苷Key words
Rheumatoid arthritis/AMPK/RANKL/OPG axis/Total glucoside of Paeoniae Radix Alba分类
医药卫生引用本文复制引用
徐婉,高雪琴,杨绍锡,顾光照,黄颖..白芍总苷调控AMPK/RANKL/OPG轴治疗CIA模型大鼠骨破坏的作用研究[J].中药材,2023,46(11):2810-2817,8.基金项目
国家自然科学基金(82060909) （82060909）
贵州省科技计划项目基金资助项目(黔科合基础-ZK(2021)一般 429) （黔科合基础-ZK(2021）
